TY - JOUR
T1 - More Efficient Media Design for Enhanced Biofouling Control in a Membrane Bioreactor
T2 - Quorum Quenching Bacteria Entrapping Hollow Cylinder
AU - Lee, Sang H.
AU - Lee, Seonki
AU - Lee, Kibaek
AU - Nahm, Chang H.
AU - Kwon, Hyeokpil
AU - Oh, Hyun Suk
AU - Won, Young June
AU - Choo, Kwang Ho
AU - Lee, Chung Hak
AU - Park, Pyung Kyu
N1 - Publisher Copyright:
© 2016 American Chemical Society.
PY - 2016/8/16
Y1 - 2016/8/16
N2 - Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.
AB - Recently, membrane bioreactors (MBRs) with quorum quenching (QQ) bacteria entrapping beads have been reported as a new paradigm in biofouling control because, unlike conventional post-biofilm control methods, bacterial QQ can inhibit biofilm formation through its combined effects of physical scouring of the membrane and inhibition of quorum sensing (QS). In this study, using a special reporter strain (Escherichia coli JB525), the interaction between QS signal molecules and quorum quenching bacteria entrapping beads (QQ-beads) was elucidated through visualization of the QS signal molecules within a QQ-bead using a fluorescence microscope. As a result, under the conditions considered in this study, the surface area of QQ-media was likely to be a dominant parameter in enhancing QQ activity over total mass of entrapped QQ bacteria because QQ bacteria located near the core of a QQ-bead were unable to display their QQ activities. On the basis of this information, a more efficient QQ-medium, a QQ hollow cylinder (QQ-HC), was designed and prepared. In batch experiments, QQ-HCs showed greater QQ activity than QQ-beads as a result of their higher surface area and enhanced physical washing effect because of their larger impact area against the membrane surface. Furthermore, it was shown that such advantages of QQ-HCs resulted in more effective mitigation of membrane fouling than from QQ-beads in lab-scale continuous MBRs.
UR - http://www.scopus.com/inward/record.url?scp=84983467377&partnerID=8YFLogxK
U2 - 10.1021/acs.est.6b01221
DO - 10.1021/acs.est.6b01221
M3 - Article
C2 - 27415662
AN - SCOPUS:84983467377
SN - 0013-936X
VL - 50
SP - 8596
EP - 8604
JO - Environmental Science and Technology
JF - Environmental Science and Technology
IS - 16
ER -