Morphological and phylogenetic characterization of tyrophagus putrescentiae schrank (sarcoptiformes: Acaridae) from hypsizygus marmoreus in Korea

The present study reports the morphological and phylogenetic characteristics of Tyrophagus putrescentiae from Hypsizygus marmoreus in Korea. This species is morphologically characterized by two pigmented eyespots on the prodorsal shield, concave posterior margin of coxal plate n, prominently and api- cally enlarged supracoxal seta shaft at bases of pectinations, and solenidion omegal on tarsi I and n, respectively and length of setae dl compared to setae cl and setae d2. The internal transcribed spacer 2 (ITS2) in the ribosomal DNA (rDNA), which is reliable for species identification, and the cytochrome oxidase sub- unit I (COl) in the mitochondrial DNA, which is a highly conserved region in eukaryotes, were sequenced. The resulting phylogenetic trees for COI and ITS2 apparently showed that the tested samples in this study were clustered with T. putrescentiae and separated from other species in the genus. This research would be useful for controlling mites from H. marmoreus in Korea and could help improve the quality and quantity of H. marmoreus production.