MPSS profiling of embryonic gonad and primordial germ cells in chicken

Heebal Kim, Tae Sub Park, Woon Kyu Lee, Sunjin Moon, Jin Nam Kim, Ji Hye Shin, Jin Gyoung Jung, Seon Duk Lee, Sang Hyun Park, Kyung Je Park, Mi A. Kim, Sang Su Shin, Tae Min Kim, Jungrye Nam, Yeonkyung Kang, Jeong Mook Lim, Jae Yong Han

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The massively parallel signature sequencing (MPSS) provides a greater depth of coverage than expressed sequence tag scan or microarray and provides a comprehensive expression profile. We used the MPSS technology to uncover gene expression profiling in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad, respectively. Using a noise distribution model, we found that 1.67% of all signatures are expressed at a higher level in PCGs and 2.81% of all signatures are expressed at a higher level in the gonad. The MPSS data are presented via an interactive web interface available at http://snugenome.snu.ac.kr/MPSS. The MPSS data have been submitted to the Gene Expression Omnibus of the National Center for Biotechnology Information (accession number GSM137300 and GSM137301 for PGCs and gonad, respectively).

Original languageEnglish
Pages (from-to)253-259
Number of pages7
JournalPhysiological Genomics
Volume29
Issue number3
DOIs
StatePublished - 27 Jun 2007

Keywords

  • Massively parallel signature sequencing

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