Multiplex minisequencing screening for PTC genotype associated with bitter taste perception

Borum Sagong; Jae Woong Bae; Mee Ra Rhyu; Un Kyung Kim; Mi Kyung Ye

doi:10.1007/s11033-013-3002-8

Multiplex minisequencing screening for PTC genotype associated with bitter taste perception

Borum Sagong, Jae Woong Bae, Mee Ra Rhyu, Un Kyung Kim, Mi Kyung Ye

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.

Original language	English
Pages (from-to)	1563-1567
Number of pages	5
Journal	Molecular Biology Reports
Volume	41
Issue number	3
DOIs	https://doi.org/10.1007/s11033-013-3002-8
State	Published - Mar 2014

Keywords

Bitter taste
Genotype
Phenylthiocarbamide (PTC)
SNaPshot minisequencing
TAS2R38 gene

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10.1007/s11033-013-3002-8

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title = "Multiplex minisequencing screening for PTC genotype associated with bitter taste perception",

abstract = "Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.",

keywords = "Bitter taste, Genotype, Phenylthiocarbamide (PTC), SNaPshot minisequencing, TAS2R38 gene",

author = "Borum Sagong and Bae, {Jae Woong} and Rhyu, {Mee Ra} and Kim, {Un Kyung} and Ye, {Mi Kyung}",

year = "2014",

month = mar,

doi = "10.1007/s11033-013-3002-8",

language = "English",

volume = "41",

pages = "1563--1567",

journal = "Molecular Biology Reports",

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T1 - Multiplex minisequencing screening for PTC genotype associated with bitter taste perception

AU - Sagong, Borum

AU - Bae, Jae Woong

AU - Rhyu, Mee Ra

AU - Kim, Un Kyung

AU - Ye, Mi Kyung

PY - 2014/3

Y1 - 2014/3

N2 - Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.

AB - Sensitivity to phenylthiocarbamide (PTC) has a bimodal distribution pattern and the genotype of the TAS2R38 gene, which is composed of combinations of three coding single nucleotide polymorphisms (SNPs), p.A49P (c.145G>C), p.V262A (c.785T>C) and p.I296 V (c.886A>G), determines the ability or inability to taste PTC. In this study, we developed a tool for genotyping of these SNPs in the TAS2R38 gene using SNaPshot minisequencing and investigated the accuracy of the tool in 100 subjects who were genotyped by Sanger sequencing. The minor allele frequencies of the three SNPs were 0.39, and these genotypes corresponded to those determined by direct sequencing. In conclusion, we successfully developed a precise and rapid genetic tool for analysis of PTC genotype associated with bitter taste perception.

KW - Bitter taste

KW - Genotype

KW - Phenylthiocarbamide (PTC)

KW - SNaPshot minisequencing

KW - TAS2R38 gene

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U2 - 10.1007/s11033-013-3002-8

DO - 10.1007/s11033-013-3002-8

M3 - Article

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AN - SCOPUS:84896042360

SN - 0301-4851

VL - 41

SP - 1563

EP - 1567

JO - Molecular Biology Reports

JF - Molecular Biology Reports

IS - 3

ER -

Multiplex minisequencing screening for PTC genotype associated with bitter taste perception

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