MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

Eun Sun Kee; Aung Htay Naing; Sun Hyung Lim; Jeung Sul Han; Chang Kil Kim

doi:10.1007/s13205-016-0396-8

MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

Eun Sun Kee, Aung Htay Naing, Sun Hyung Lim, Jeung Sul Han, Chang Kil Kim

Department of Horticultural Science

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

Original language	English
Article number	79
Pages (from-to)	1-8
Number of pages	8
Journal	3 Biotech
Volume	6
Issue number	1
DOIs	https://doi.org/10.1007/s13205-016-0396-8
State	Published - 1 Dec 2016

Keywords

Anthocyanin
RT-PCR
Spectrophotometer
Transcription factor

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title = "MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars",

abstract = "A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars {\textquoteleft}Peach ND{\textquoteright}, {\textquoteleft}Peach Red{\textquoteright}, and {\textquoteleft}Vivid Scarlet{\textquoteright} under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.",

keywords = "Anthocyanin, RT-PCR, Spectrophotometer, Transcription factor",

author = "Kee, {Eun Sun} and Naing, {Aung Htay} and Lim, {Sun Hyung} and Han, {Jeung Sul} and Kim, {Chang Kil}",

note = "Publisher Copyright: {\textcopyright} 2016, The Author(s).",

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doi = "10.1007/s13205-016-0396-8",

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T1 - MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

AU - Kee, Eun Sun

AU - Naing, Aung Htay

AU - Lim, Sun Hyung

AU - Han, Jeung Sul

AU - Kim, Chang Kil

PY - 2016/12/1

Y1 - 2016/12/1

N2 - A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

AB - A MYB transcription factor gene, RsMYB1, from radish was introduced into the chrysanthemum cultivars ‘Peach ND’, ‘Peach Red’, and ‘Vivid Scarlet’ under the control of the cauliflower mosaic virus 35S promoter. Presence of RsMYB1 in transgenic lines was confirmed using polymerase chain reaction (PCR). Results of reverse-transcription-PCR analysis revealed that the expression of RsMYB1 was stable in all transgenic lines and could enhance the expression levels of three key biosynthetic genes (F3H, DFR, and ANS) involved in anthocyanin production. Accordingly, anthocyanin content was significantly higher in transgenic lines than in control of all the cultivars, although the increasement was not visually observed in any of the transgenic lines. Therefore, these results demonstrate that RsMYB1 has potential to enhance anthocyanin content in the chrysanthemums.

KW - Anthocyanin

KW - RT-PCR

KW - Spectrophotometer

KW - Transcription factor

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JO - 3 Biotech

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ER -

MYB transcription factor isolated from Raphanus sativus enhances anthocyanin accumulation in chrysanthemum cultivars

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Keywords

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