TY - JOUR
T1 - Neolignans from the fruits of Magnolia obovata and their inhibition effect on NO production in LPS-induced RAW 2647 cells
AU - Seo, Kyeong Hwa
AU - Lee, Dae Young
AU - Lee, Dong Sung
AU - Park, Ji Hae
AU - Jeong, Rak Hun
AU - Jung, Ye Jin
AU - Shrestha, Sabina
AU - Chung, In Sik
AU - Kim, Geum Soog
AU - Kim, Youn Chul
AU - Baek, Nam In
PY - 2013
Y1 - 2013
N2 - Three new neolignans, named 9-methoxyobovatol (6), magnobovatol (7), and 2-hydroxyobovaaldehyde (9), along with six known ones, magnolol (1), honokiol (2), isomagnolol (3), obovatol (4), obovatal (5), and obovaaldehyde (8), were isolated from the fruits of Magnolia obovata using silica gel and ODS column chromatography. From the results of spectroscopic data including EIMS, IR, 1H- and 13C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC), the chemical structures were determined. All isolated compounds were evaluated for inhibition activity on nitric oxide production in LPS-induced RAW 264.7 cells, and compounds 1-4, 6, 7, and 9 showed significant activity with IC 50 values of 15.8 ± 0.3, 3.3 ± 1.2, 14.1 ± 0.9, 6.2 ± 1.2, 14.8 ± 2.3, 14.2 ± 1.2, and 14.8 ± 3.2 μM, respectively, without any visible toxic effect.
AB - Three new neolignans, named 9-methoxyobovatol (6), magnobovatol (7), and 2-hydroxyobovaaldehyde (9), along with six known ones, magnolol (1), honokiol (2), isomagnolol (3), obovatol (4), obovatal (5), and obovaaldehyde (8), were isolated from the fruits of Magnolia obovata using silica gel and ODS column chromatography. From the results of spectroscopic data including EIMS, IR, 1H- and 13C-NMR, DEPT, and 2D-NMR (gCOSY, gHSQC, gHMBC), the chemical structures were determined. All isolated compounds were evaluated for inhibition activity on nitric oxide production in LPS-induced RAW 264.7 cells, and compounds 1-4, 6, 7, and 9 showed significant activity with IC 50 values of 15.8 ± 0.3, 3.3 ± 1.2, 14.1 ± 0.9, 6.2 ± 1.2, 14.8 ± 2.3, 14.2 ± 1.2, and 14.8 ± 3.2 μM, respectively, without any visible toxic effect.
KW - 9-methoxyobovatol
KW - anti-inflammation
KW - magnobovatol
KW - Magnolia obovata
KW - Magnoliaceae
KW - neolignan
KW - NO production
UR - http://www.scopus.com/inward/record.url?scp=84885057258&partnerID=8YFLogxK
U2 - 10.1055/s-0033-1350673
DO - 10.1055/s-0033-1350673
M3 - Article
C2 - 23970426
AN - SCOPUS:84885057258
SN - 0032-0943
VL - 79
SP - 1335
EP - 1340
JO - Planta Medica
JF - Planta Medica
IS - 14
ER -