Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

Zae Young Ryoo; Boo Kyoung Jung; Sang Ryeul Lee; Myoung Ok Kim; Sung Hyun Kim; Hyo Jin Kim; Jung Yong Ahn; Tae Hoon Lee; Youl Hee Cho; Jae Hak Park; Jin Kyeoung Kim

doi:10.1016/j.bbrc.2006.08.137

Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

Zae Young Ryoo, Boo Kyoung Jung, Sang Ryeul Lee, Myoung Ok Kim, Sung Hyun Kim, Hyo Jin Kim, Jung Yong Ahn, Tae Hoon Lee, Youl Hee Cho, Jae Hak Park, Jin Kyeoung Kim

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12 Scopus citations

Abstract

Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.

Original language	English
Pages (from-to)	995-1002
Number of pages	8
Journal	Biochemical and Biophysical Research Communications
Volume	349
Issue number	3
DOIs	https://doi.org/10.1016/j.bbrc.2006.08.137
State	Published - 27 Oct 2006

Keywords

Fibroblast
IMUP-1
IMUP-2
SV40
Tumorigenicity

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.bbrc.2006.08.137

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Ryoo, Z. Y., Jung, B. K., Lee, S. R., Kim, M. O., Kim, S. H., Kim, H. J., Ahn, J. Y., Lee, T. H., Cho, Y. H., Park, J. H., & Kim, J. K. (2006). Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts. Biochemical and Biophysical Research Communications, 349(3), 995-1002. https://doi.org/10.1016/j.bbrc.2006.08.137

@article{15a994ae96a74efaab2516e76fd3581d,

title = "Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts",

abstract = "Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.",

keywords = "Fibroblast, IMUP-1, IMUP-2, SV40, Tumorigenicity",

author = "Ryoo, {Zae Young} and Jung, {Boo Kyoung} and Lee, {Sang Ryeul} and Kim, {Myoung Ok} and Kim, {Sung Hyun} and Kim, {Hyo Jin} and Ahn, {Jung Yong} and Lee, {Tae Hoon} and Cho, {Youl Hee} and Park, {Jae Hak} and Kim, {Jin Kyeoung}",

year = "2006",

month = oct,

day = "27",

doi = "10.1016/j.bbrc.2006.08.137",

language = "English",

volume = "349",

pages = "995--1002",

journal = "Biochemical and Biophysical Research Communications",

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Ryoo, ZY, Jung, BK, Lee, SR, Kim, MO, Kim, SH, Kim, HJ, Ahn, JY, Lee, TH, Cho, YH, Park, JH & Kim, JK 2006, 'Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts', Biochemical and Biophysical Research Communications, vol. 349, no. 3, pp. 995-1002. https://doi.org/10.1016/j.bbrc.2006.08.137

TY - JOUR

T1 - Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

AU - Ryoo, Zae Young

AU - Jung, Boo Kyoung

AU - Lee, Sang Ryeul

AU - Kim, Myoung Ok

AU - Kim, Sung Hyun

AU - Kim, Hyo Jin

AU - Ahn, Jung Yong

AU - Lee, Tae Hoon

AU - Cho, Youl Hee

AU - Park, Jae Hak

AU - Kim, Jin Kyeoung

PY - 2006/10/27

Y1 - 2006/10/27

N2 - Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.

AB - Immortalization-upregulated protein 1 (IMUP-1) and immortalization-upregulated protein 2 (IMUP-2) genes have been recently cloned and are known to be involved in SV40-mediated immortalization. IMUP-1 and IMUP-2 genes were strongly expressed in various cancer cell lines and tumors, suggesting the possibility that they might be involved in tumorigenicity. To directly elucidate the functional role of IMUP-1 and IMUP-2 on neoplastic transformation and tumorigenicity, we stably transfected IMUP-1 and IMUP-2 into NIH/3T3 mouse fibroblast cells. Cellular characteristics of the neoplastic transformation were assessed by transformation foci, growth in soft agar, and tumor development in nude mice. We found that IMUP-1 and IMUP-2 overexpressing cells showed altered growth properties, anchorage-independent growth in soft agar and inducing tumor in nude mice. Furthermore, IMUP-1 and IMUP-2 transformants proliferated in reduced serum and shortened cell cycle. These results suggest that ectopic overexpression of IMUP-1 and IMUP-2 may play an important role in acquiring a transformed phenotype, tumorigenicity in vivo, and be related to cellular proliferation.

KW - Fibroblast

KW - IMUP-1

KW - IMUP-2

KW - SV40

KW - Tumorigenicity

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U2 - 10.1016/j.bbrc.2006.08.137

DO - 10.1016/j.bbrc.2006.08.137

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AN - SCOPUS:33748592846

SN - 0006-291X

VL - 349

SP - 995

EP - 1002

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

IS - 3

ER -

Neoplastic transformation and tumorigenesis associated with overexpression of imup-1 and imup-2 genes in cultured NIH/3T3 mouse fibroblasts

Abstract

Keywords

UN SDGs

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