New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model

Prakash Gangadaran; Xiu Juan Li; Senthil kumar Kalimuthu; Oh Ji Min; Chae Moon Hong; Ramya Lakshmi Rajendran; Ho Won Lee; Liya Zhu; Se Hwan Baek; Shin Young Jeong; Sang Woo Lee; Jaetae Lee; Byeong Cheol Ahn

doi:10.1038/s41598-018-31998-y

New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model

Prakash Gangadaran, Xiu Juan Li, Senthil kumar Kalimuthu, Oh Ji Min, Chae Moon Hong, Ramya Lakshmi Rajendran, Ho Won Lee, Liya Zhu, Se Hwan Baek, Shin Young Jeong, Sang Woo Lee, Jaetae Lee, Byeong Cheol Ahn

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Abstract

Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.

Original language	English
Article number	13509
Journal	Scientific Reports
Volume	8
Issue number	1
DOIs	https://doi.org/10.1038/s41598-018-31998-y
State	Published - 1 Dec 2018

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Gangadaran, P., Li, X. J., Kalimuthu, S. K., Min, O. J., Hong, C. M., Rajendran, R. L., Lee, H. W., Zhu, L., Baek, S. H., Jeong, S. Y., Lee, S. W., Lee, J., & Ahn, B. C. (2018). New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model. Scientific Reports, 8(1), Article 13509. https://doi.org/10.1038/s41598-018-31998-y

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abstract = "Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.",

author = "Prakash Gangadaran and Li, {Xiu Juan} and Kalimuthu, {Senthil kumar} and Min, {Oh Ji} and Hong, {Chae Moon} and Rajendran, {Ramya Lakshmi} and Lee, {Ho Won} and Liya Zhu and Baek, {Se Hwan} and Jeong, {Shin Young} and Lee, {Sang Woo} and Jaetae Lee and Ahn, {Byeong Cheol}",

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doi = "10.1038/s41598-018-31998-y",

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Gangadaran, P, Li, XJ, Kalimuthu, SK, Min, OJ, Hong, CM, Rajendran, RL, Lee, HW, Zhu, L, Baek, SH, Jeong, SY, Lee, SW, Lee, J & Ahn, BC 2018, 'New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model', Scientific Reports, vol. 8, no. 1, 13509. https://doi.org/10.1038/s41598-018-31998-y

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AU - Li, Xiu Juan

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AU - Min, Oh Ji

AU - Hong, Chae Moon

AU - Rajendran, Ramya Lakshmi

AU - Lee, Ho Won

AU - Zhu, Liya

AU - Baek, Se Hwan

AU - Jeong, Shin Young

AU - Lee, Sang Woo

AU - Lee, Jaetae

AU - Ahn, Byeong Cheol

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.

AB - Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.

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New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model

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