New Optical Imaging Reporter-labeled Anaplastic Thyroid Cancer-Derived Extracellular Vesicles as a Platform for In Vivo Tumor Targeting in a Mouse Model

Prakash Gangadaran, Xiu Juan Li, Senthil kumar Kalimuthu, Oh Ji Min, Chae Moon Hong, Ramya Lakshmi Rajendran, Ho Won Lee, Liya Zhu, Se Hwan Baek, Shin Young Jeong, Sang Woo Lee, Jaetae Lee, Byeong Cheol Ahn

Research output: Contribution to journalArticlepeer-review

19 Scopus citations

Abstract

Extracellular vesicles (EVs), originating from multivesicular bodies by invagination of the endosomal membrane, are communication channels between distant cells. They are natural carriers of exogeneous cellular materials and have been exploited as drug delivery carriers in various diseases. Here, we found that tumor cell-derived EVs can be used as efficient targets in tumors by monitoring with an optical reporter system. Anaplastic thyroid cancer (CAL62) cell-derived EVs with Renilla luciferase (Rluc) were used to target CAL62 tumors in a mouse model. Optical imaging revealed that cancer cell-derived EVs (EV-CAL62/Rluc) targeted the original tumor (CAL62) in mice within 30 min after systemic injection. Furthermore, fluorescence imaging revealed that EV-CAL62/Rluc were internalized into CAL62 tumors in the mice. Ex vivo Optical imaging further confirmed the in vivo finding. Here, we successfully monitored the tumor targeting ability of tumor cell-derived EVs by optical imaging. Based on these results, tumor cell-derived EVs are highly effective natural carriers for drug delivery for cancer therapies.

Original languageEnglish
Article number13509
JournalScientific Reports
Volume8
Issue number1
DOIs
StatePublished - 1 Dec 2018

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