Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid

Suhui Jeong; Yeji Jeon; Jaehun Mun; Se Min Jeong; Huiling Liang; Kyeongwoon Chung; Pyong In Yi; Beum Soo An; Sungbaek Seo

doi:10.3390/chemosensors11010049

Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid

Suhui Jeong, Yeji Jeon, Jaehun Mun, Se Min Jeong, Huiling Liang, Kyeongwoon Chung, Pyong In Yi, Beum Soo An, Sungbaek Seo

Department of Biofibers and Biomaterials Science

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann’s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM.

Original language	English
Article number	49
Journal	Chemosensors
Volume	11
Issue number	1
DOIs	https://doi.org/10.3390/chemosensors11010049
State	Published - Jan 2023

Keywords

microcapsules
natural free amino acid
ninhydrin
theanine

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10.3390/chemosensors11010049

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title = "Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid",

abstract = "Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann{\textquoteright}s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM.",

keywords = "microcapsules, natural free amino acid, ninhydrin, theanine",

author = "Suhui Jeong and Yeji Jeon and Jaehun Mun and Jeong, {Se Min} and Huiling Liang and Kyeongwoon Chung and Yi, {Pyong In} and An, {Beum Soo} and Sungbaek Seo",

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doi = "10.3390/chemosensors11010049",

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T1 - Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid

AU - Jeong, Suhui

AU - Jeon, Yeji

AU - Mun, Jaehun

AU - Jeong, Se Min

AU - Liang, Huiling

AU - Chung, Kyeongwoon

AU - Yi, Pyong In

AU - An, Beum Soo

AU - Seo, Sungbaek

PY - 2023/1

Y1 - 2023/1

N2 - Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann’s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM.

AB - Natural free amino acids present in plant extracts or tea infusions provide a unique flavor and potential effect on anxiety and blood pressure reduction. Accordingly, quantifying free amino acids in foods has been of interest to food science and analytical research fields. The ninhydrin solution-based assay is a colorimetric method based on the formation and detection of Ruhemann’s purple complex. Media-based colorimetric detection requires specialized facilities and personnel; moreover, it can suffer from the interference of the analyte color. In this study, we developed ninhydrin-loaded microcapsules and a simple free amino acids detection procedure, by simply dipping the microcapsules into the analyte solution for 3 min. Among the five tested natural free amino acids, theanine exhibited the highest colorimetric response to microcapsule-based detection, with a limit of detection of 0.826 mM.

KW - microcapsules

KW - natural free amino acid

KW - ninhydrin

KW - theanine

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Ninhydrin Loaded Microcapsules for Detection of Natural Free Amino Acid

Abstract

Keywords

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