Noble 3,4-seco-triterpenoid glycosides from the fruits of acanthopanax sessiliflorus and their anti-neuroinflammatory effects

Bo Ram Choi, Hyoung Geun Kim, Wonmin Ko, Linsha Dong, Dahye Yoon, Seon Min Oh, Young Seob Lee, Dong Sung Lee, Nam In Baek, Dae Young Lee

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Acanthopanax sessiliflorus (Araliaceae) have been reported to exhibit many pharmacological activities. Our preliminary study suggested that A. sessiliflorus fruits include many bioactive 3,4-secotriterpenoids. A. sessiliflorus fruits were extracted in aqueous EtOH and fractionated into EtOAc, n-BuOH, and H2 O fractions. Repeated column chromatographies for the organic fractions led to the isolation of 3,4-seco-triterpenoid glycosides, including new compounds. Ultra-high-performance liquid chromatography (UPLC) mass spectrometry (MS) systems were used for quantitation and quantification. BV2 and RAW264.7 cells were induced by LPS, and the levels of pro-inflammatory cytokines and mediators and their underlying mechanisms were measured by ELISA and Western blotting. NMR, IR, and HR-MS analyses revealed the chemical structures of the nine noble 3,4seco-triterpenoid glycosides, acanthosessilioside G–O, and two known ones. The amounts of the compounds were 0.01–2.806 mg/g, respectively. Acanthosessilioside K, L, and M were the most effective in inhibiting NO, PGE2, TNF-α, IL-1β, and IL-6 production and reducing iNOS and COX-2 expression. In addition, it had inhibitory effects on the LPS-induced p38 and ERK MAPK phosphorylation in both BV2 and RAW264.7 cells. Nine noble 3,4-seco-triterpenoid glycosides were isolated from A. sessiliflorus fruits, and acanthosessilioside K, L, and M showed high anti-inflammatory and anti-neuroinflammatory effects.

Original languageEnglish
Article number1334
JournalAntioxidants
Volume10
Issue number9
DOIs
StatePublished - Sep 2021

Keywords

  • Acanthopanax sessiliflorus fruit
  • Acanthosessiliosides
  • Anti-inflammation
  • Anti-neuroinflammation
  • NMR
  • Quantification

Fingerprint

Dive into the research topics of 'Noble 3,4-seco-triterpenoid glycosides from the fruits of acanthopanax sessiliflorus and their anti-neuroinflammatory effects'. Together they form a unique fingerprint.

Cite this