Non-Biofouling Performance and Binding Capabilities of Amylose Film Coated on Glass Surface

We demonstrated the non-biofouling performances and binding capabilities of amylose film coated on a glass surface using fluorescence analysis. The amylose film was simply prepared using a drop-casting method and then functionalized with amine-terminated biotin through surface organic reactions such as periodate oxidation, amide bond formation, and reduction. The biotin, spatially patterned on the film, selectively interacted with streptavidin labeled with a dye, which enabled the evaluation of the binding capabilities and non-biofouling efficacy of 0.2 and 0.4 wt% amylose film. These films exhibited similar results, such as excellent non-biofouling properties and slightly low binding capabilities (minimum concentration of biotin: 10 μM).