Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers

Choong Jae Lee; Eun Young Yoo; Joo Hyun Shin; Jemin Lee; Hee Sook Hwang; Byung Dong Kim

Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers

Choong Jae Lee, Eun Young Yoo, Joo Hyun Shin, Jemin Lee, Hee Sook Hwang, Byung Dong Kim

Department of Horticultural Science

Seoul National University

Research output: Contribution to journal › Article › peer-review

57 Scopus citations

Abstract

The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 F₂ individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5′ upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.

Original language	English
Pages (from-to)	262-267
Number of pages	6
Journal	Molecules and Cells
Volume	19
Issue number	2
State	Published - 2005

Keywords

Capsaicinoid Synthetase (CS)
Capsicum annuum
Pungency
Sequence- characterized Amplified Regions (SCAR) Marker

Cite this

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title = "Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers",

abstract = "The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 F2 individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5′ upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.",

keywords = "Capsaicinoid Synthetase (CS), Capsicum annuum, Pungency, Sequence- characterized Amplified Regions (SCAR) Marker",

author = "Lee, {Choong Jae} and Yoo, {Eun Young} and Shin, {Joo Hyun} and Jemin Lee and Hwang, {Hee Sook} and Kim, {Byung Dong}",

year = "2005",

language = "English",

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journal = "Molecules and Cells",

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T1 - Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers

AU - Lee, Choong Jae

AU - Yoo, Eun Young

AU - Shin, Joo Hyun

AU - Lee, Jemin

AU - Hwang, Hee Sook

AU - Kim, Byung Dong

PY - 2005

Y1 - 2005

N2 - The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 F2 individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5′ upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.

AB - The capsaicinoid synthetase (CS) gene cosegregated perfectly with the C locus, which controls the presence of pungency, in 121 F2 individuals from a cross between 'ECW123R' and 'CM334', both of Capsicum annuum. We concluded that CS and C are tightly linked. Sequence analysis of the genes of four pungent and four non-pungent pepper lines showed that the non-pungent peppers had a 2,529 bp-deletion in the 5′ upstream region of CS. We have developed molecular markers of the C locus to detect pungency at the seedling stage. Based on the deleted sequence, we developed five SCAR markers, two of them being codominant. These SCAR markers will be useful for easy, accurate, and early detection of non-pungent individuals in breeding programs.

KW - Capsaicinoid Synthetase (CS)

KW - Capsicum annuum

KW - Pungency

KW - Sequence- characterized Amplified Regions (SCAR) Marker

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M3 - Article

C2 - 15879712

AN - SCOPUS:26044463205

SN - 1016-8478

VL - 19

SP - 262

EP - 267

JO - Molecules and Cells

JF - Molecules and Cells

IS - 2

ER -

Non-pungent Capsicum contains a deletion in the capsaicinoid synthetase gene, which allows early detection of pungency with SCAR markers

Abstract

Keywords

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