Noninvasive measurement of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells using near-infrared spectroscopy

Ki Taek Lim, Tu San Park, Jang Ho Kim, Pill Hoon Choung, Jong Hoon Chung

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

On-line monitoring and control of cell culture is important for optimal and consistent biological activities. Glucose is a major carbon and energy source for cultured cells, but glycolytic metabolism often results in the accumulation of lactate. Measurements of glucose and lactate levels are therefore useful for determining metabolic activities in cell culture. In this work, a method for the noninvasive analysis of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells (hABMSCs) was developed using near-infrared spectroscopy (NIRS). Partial least square regression (PLSR) was used to calculate and validate an adaptive multivariable calibration model based on NIR spectra of the culture media in order to determine levels of glucose uptake and lactate production. Our results showed a strong inverse relationship between glucose consumption and lactate accumulation. Glucose concentrations dropped from about 1,400 ppm to 100 ppm for seven days, while lactate levels rose up from approximately 0.1 ppm to 1.4 ppm during the same time period. The NIR spectra of samples in this study indicated that lactate had absorption peaks at 1,675, 1,690 and 1,730 nm, while glucose absorbed at 1,613, 1,689, and 1732 nm. The coefficients of determination (R2) for glucose and lactate in the regression data were 0.992 and 0.950, respectively. Likewise, the coefficients of determination for pH and culture days were 0.991 and 0.921, respectively. The results of this study demonstrate that NIRS can be applied for the noninvasive measurement of critical nutrients and byproducts in culture media of hABMSCs.

Original languageEnglish
Pages (from-to)503-511
Number of pages9
JournalTissue Engineering and Regenerative Medicine
Volume8
Issue number6
StatePublished - Nov 2011

Keywords

  • Glucose
  • Human alveolar bone-derived mesenchymal stem cells
  • Lactate
  • Metabolites
  • Near-infrared spectroscopy

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