TY - JOUR
T1 - Noninvasive measurement of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells using near-infrared spectroscopy
AU - Lim, Ki Taek
AU - Park, Tu San
AU - Kim, Jang Ho
AU - Choung, Pill Hoon
AU - Chung, Jong Hoon
PY - 2011/11
Y1 - 2011/11
N2 - On-line monitoring and control of cell culture is important for optimal and consistent biological activities. Glucose is a major carbon and energy source for cultured cells, but glycolytic metabolism often results in the accumulation of lactate. Measurements of glucose and lactate levels are therefore useful for determining metabolic activities in cell culture. In this work, a method for the noninvasive analysis of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells (hABMSCs) was developed using near-infrared spectroscopy (NIRS). Partial least square regression (PLSR) was used to calculate and validate an adaptive multivariable calibration model based on NIR spectra of the culture media in order to determine levels of glucose uptake and lactate production. Our results showed a strong inverse relationship between glucose consumption and lactate accumulation. Glucose concentrations dropped from about 1,400 ppm to 100 ppm for seven days, while lactate levels rose up from approximately 0.1 ppm to 1.4 ppm during the same time period. The NIR spectra of samples in this study indicated that lactate had absorption peaks at 1,675, 1,690 and 1,730 nm, while glucose absorbed at 1,613, 1,689, and 1732 nm. The coefficients of determination (R2) for glucose and lactate in the regression data were 0.992 and 0.950, respectively. Likewise, the coefficients of determination for pH and culture days were 0.991 and 0.921, respectively. The results of this study demonstrate that NIRS can be applied for the noninvasive measurement of critical nutrients and byproducts in culture media of hABMSCs.
AB - On-line monitoring and control of cell culture is important for optimal and consistent biological activities. Glucose is a major carbon and energy source for cultured cells, but glycolytic metabolism often results in the accumulation of lactate. Measurements of glucose and lactate levels are therefore useful for determining metabolic activities in cell culture. In this work, a method for the noninvasive analysis of glucose and lactate metabolism of human alveolar bone-derived mesenchymal stem cells (hABMSCs) was developed using near-infrared spectroscopy (NIRS). Partial least square regression (PLSR) was used to calculate and validate an adaptive multivariable calibration model based on NIR spectra of the culture media in order to determine levels of glucose uptake and lactate production. Our results showed a strong inverse relationship between glucose consumption and lactate accumulation. Glucose concentrations dropped from about 1,400 ppm to 100 ppm for seven days, while lactate levels rose up from approximately 0.1 ppm to 1.4 ppm during the same time period. The NIR spectra of samples in this study indicated that lactate had absorption peaks at 1,675, 1,690 and 1,730 nm, while glucose absorbed at 1,613, 1,689, and 1732 nm. The coefficients of determination (R2) for glucose and lactate in the regression data were 0.992 and 0.950, respectively. Likewise, the coefficients of determination for pH and culture days were 0.991 and 0.921, respectively. The results of this study demonstrate that NIRS can be applied for the noninvasive measurement of critical nutrients and byproducts in culture media of hABMSCs.
KW - Glucose
KW - Human alveolar bone-derived mesenchymal stem cells
KW - Lactate
KW - Metabolites
KW - Near-infrared spectroscopy
UR - http://www.scopus.com/inward/record.url?scp=84884612963&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:84884612963
SN - 1738-2696
VL - 8
SP - 503
EP - 511
JO - Tissue Engineering and Regenerative Medicine
JF - Tissue Engineering and Regenerative Medicine
IS - 6
ER -