Novel antibiotics regeneration and genetic transformation with RsMYB1 gene of recalcitrant chrysanthemum cv. Shinma

A. H. Naing, T. Ngoc Ai, S. M. Jeon, K. I. Park, S. H. Lim, K. B. Lim, Chang Kil Kim

Research output: Contribution to journalArticlepeer-review

5 Scopus citations

Abstract

This research was conducted to develop genetic transformation of the recalcitrant chrysanthemum cv. Shinma by application of appropriate antibiotics and selective agents. Clavamox had the least inhibitory effect on shoot regeneration compared to timentin, carbenicillin, and cefotaxime. Clavamox, at a concentration of 125 mg L−1, was found to be the most suitable for shoot regeneration and production of quality shoots, suppressing the growth of Agrobacterium in explants infected with strains GV3101 or C58C1 for 3 and 4 weeks, respectively. The concentration of phosphinothricin (PPT) was found to be 1.0 mg L−1 for screening of putative transgenic shoots. Moreover, transgenic chrysanthemums were obtained by culturing explants co-cultivated with A. tumefaciens strain GV3101 harboring an anthocyanin regulatory gene RsMYB1 isolated from radish (Raphanus sativus), which was placed under the control of cauliflower mosaic virus promoter (CaMV) 35S and petal-specific promoter InMYB1 isolated from the morning glory (Ipomoea nil), on shoot regeneration medium supplemented with recommended concentration of antibiotic and selective agent. Flow cytometry analysis revealed that there was no variation in ploidy level between transgenic plants and donor plants (non-transformants). To our knowledge, this is the first report of the use of Clavamox and MYB transcription factor for genetic transformation of this chrysanthemum.

Original languageEnglish
Pages (from-to)98-107
Number of pages10
JournalPlant Biosystems
Volume151
Issue number1
DOIs
StatePublished - 9 Jan 2017

Keywords

  • Anthocyanin
  • Antibiotic
  • Ploidy level
  • Selection marker
  • Transgenic plant

Fingerprint

Dive into the research topics of 'Novel antibiotics regeneration and genetic transformation with RsMYB1 gene of recalcitrant chrysanthemum cv. Shinma'. Together they form a unique fingerprint.

Cite this