NUCB1 is required for proper insulin signaling to control longevity in Drosophila

Jong Won Yoon; Si Eun Baek; Jae Yoon Yang; Eunbyul Yeom

doi:10.1111/ggi.14858

NUCB1 is required for proper insulin signaling to control longevity in Drosophila

Jong Won Yoon, Si Eun Baek, Jae Yoon Yang, Eunbyul Yeom

Kyungpook National University

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Aim: We examined the novel role of NUCB1(Nucleobindin-1) associated with longevity in Drosophila melanogaster. Methods: We measured the lifespan, metabolic phenotypes, and mRNA levels of Drosophila insulin-like peptides (Dilps), the protein level of phosphorylated AKT, and the localization of FOXO and its target gene expressions in the NUCB1 knockdown condition. Results: NUCB1 knockdown flies show an extended lifespan and metabolic phenotypes such as increased circulating glucose level and starvation resistance. The mRNA expression levels of Dilps and the protein level of phosphorylated AKT, a downstream component of insulin signaling, were decreased in NUCB1 knockdown flies compared with the control flies. Also, the nuclear localization of FOXO and its target gene expressions, such as d4E-BP and InR, were elevated. Conclusions: The results show that NUCB1 knockdown flies exhibits an extended lifespan. These findings suggest that NUCB1 modulates longevity through insulin signaling in Drosophila. Geriatr Gerontol Int 2024; 24: 486–492.

Original language	English
Pages (from-to)	486-492
Number of pages	7
Journal	Geriatrics and Gerontology International
Volume	24
Issue number	5
DOIs	https://doi.org/10.1111/ggi.14858
State	Published - May 2024

Keywords

Dilps
Drosophila melanogaster
NUCB1
insulin signaling
longevity

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10.1111/ggi.14858

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title = "NUCB1 is required for proper insulin signaling to control longevity in Drosophila",

abstract = "Aim: We examined the novel role of NUCB1(Nucleobindin-1) associated with longevity in Drosophila melanogaster. Methods: We measured the lifespan, metabolic phenotypes, and mRNA levels of Drosophila insulin-like peptides (Dilps), the protein level of phosphorylated AKT, and the localization of FOXO and its target gene expressions in the NUCB1 knockdown condition. Results: NUCB1 knockdown flies show an extended lifespan and metabolic phenotypes such as increased circulating glucose level and starvation resistance. The mRNA expression levels of Dilps and the protein level of phosphorylated AKT, a downstream component of insulin signaling, were decreased in NUCB1 knockdown flies compared with the control flies. Also, the nuclear localization of FOXO and its target gene expressions, such as d4E-BP and InR, were elevated. Conclusions: The results show that NUCB1 knockdown flies exhibits an extended lifespan. These findings suggest that NUCB1 modulates longevity through insulin signaling in Drosophila. Geriatr Gerontol Int 2024; 24: 486–492.",

keywords = "Dilps, Drosophila melanogaster, NUCB1, insulin signaling, longevity",

author = "Yoon, {Jong Won} and Baek, {Si Eun} and Yang, {Jae Yoon} and Eunbyul Yeom",

note = "Publisher Copyright: {\textcopyright} 2024 Japan Geriatrics Society.",

year = "2024",

month = may,

doi = "10.1111/ggi.14858",

language = "English",

volume = "24",

pages = "486--492",

journal = "Geriatrics and Gerontology International",

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T1 - NUCB1 is required for proper insulin signaling to control longevity in Drosophila

AU - Yoon, Jong Won

AU - Baek, Si Eun

AU - Yang, Jae Yoon

AU - Yeom, Eunbyul

PY - 2024/5

Y1 - 2024/5

N2 - Aim: We examined the novel role of NUCB1(Nucleobindin-1) associated with longevity in Drosophila melanogaster. Methods: We measured the lifespan, metabolic phenotypes, and mRNA levels of Drosophila insulin-like peptides (Dilps), the protein level of phosphorylated AKT, and the localization of FOXO and its target gene expressions in the NUCB1 knockdown condition. Results: NUCB1 knockdown flies show an extended lifespan and metabolic phenotypes such as increased circulating glucose level and starvation resistance. The mRNA expression levels of Dilps and the protein level of phosphorylated AKT, a downstream component of insulin signaling, were decreased in NUCB1 knockdown flies compared with the control flies. Also, the nuclear localization of FOXO and its target gene expressions, such as d4E-BP and InR, were elevated. Conclusions: The results show that NUCB1 knockdown flies exhibits an extended lifespan. These findings suggest that NUCB1 modulates longevity through insulin signaling in Drosophila. Geriatr Gerontol Int 2024; 24: 486–492.

AB - Aim: We examined the novel role of NUCB1(Nucleobindin-1) associated with longevity in Drosophila melanogaster. Methods: We measured the lifespan, metabolic phenotypes, and mRNA levels of Drosophila insulin-like peptides (Dilps), the protein level of phosphorylated AKT, and the localization of FOXO and its target gene expressions in the NUCB1 knockdown condition. Results: NUCB1 knockdown flies show an extended lifespan and metabolic phenotypes such as increased circulating glucose level and starvation resistance. The mRNA expression levels of Dilps and the protein level of phosphorylated AKT, a downstream component of insulin signaling, were decreased in NUCB1 knockdown flies compared with the control flies. Also, the nuclear localization of FOXO and its target gene expressions, such as d4E-BP and InR, were elevated. Conclusions: The results show that NUCB1 knockdown flies exhibits an extended lifespan. These findings suggest that NUCB1 modulates longevity through insulin signaling in Drosophila. Geriatr Gerontol Int 2024; 24: 486–492.

KW - Dilps

KW - Drosophila melanogaster

KW - NUCB1

KW - insulin signaling

KW - longevity

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JO - Geriatrics and Gerontology International

JF - Geriatrics and Gerontology International

IS - 5

ER -

NUCB1 is required for proper insulin signaling to control longevity in Drosophila

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