Occurrence of SDR 2N-gametes in Lilium hybrids

Ki Byung Lim; Tsai Mu Shen; Rodrigo Barba-Gonzalez; M. S. Ramanna; Jaap M. Van Tuyl

doi:10.1270/jsbbs.54.13

Occurrence of SDR 2N-gametes in Lilium hybrids

Ki Byung Lim, Tsai Mu Shen, Rodrigo Barba-Gonzalez, M. S. Ramanna, Jaap M. Van Tuyl

Department of Horticultural Science

Research output: Contribution to journal › Article › peer-review

31 Scopus citations

Abstract

The mechanism of SDR 2n-pollen formation was analyzed in two intra-sectional diploid (2n = 2x = 24) Lilium hybrids (Enchantment x L. pumilum). Variable frequencies of 2n-pollen were found. Meiotic analysis indicated that the intra-sectional hybrids showed perfect chromosome pairing in most cases at metaphase I and normal anaphase I movement of pollen mother cells (PMCs), but produced 2n-pollen by second division restitution (SDR). A high bivalent formation (11.9II and 11.8II, respectively) at metaphase I, irregular meiotic division such as unbalanced chromosome separation and chromatic fragmentation resulted yet in acceptable pollen fertility for cross-pollination. The hybrids were fertile, and when used as male parents, offspring could be generated. The significance of the occurrence of 2n-pollen for the breeding of lilies was analyzed.

Original language	English
Pages (from-to)	13-18
Number of pages	6
Journal	Breeding Science
Volume	54
Issue number	1
DOIs	https://doi.org/10.1270/jsbbs.54.13
State	Published - Mar 2004

Keywords

Intra-sectional hybrid
Meiosis
Pollen viability
SDR
Sexual polyploid

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10.1270/jsbbs.54.13

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title = "Occurrence of SDR 2N-gametes in Lilium hybrids",

abstract = "The mechanism of SDR 2n-pollen formation was analyzed in two intra-sectional diploid (2n = 2x = 24) Lilium hybrids (Enchantment x L. pumilum). Variable frequencies of 2n-pollen were found. Meiotic analysis indicated that the intra-sectional hybrids showed perfect chromosome pairing in most cases at metaphase I and normal anaphase I movement of pollen mother cells (PMCs), but produced 2n-pollen by second division restitution (SDR). A high bivalent formation (11.9II and 11.8II, respectively) at metaphase I, irregular meiotic division such as unbalanced chromosome separation and chromatic fragmentation resulted yet in acceptable pollen fertility for cross-pollination. The hybrids were fertile, and when used as male parents, offspring could be generated. The significance of the occurrence of 2n-pollen for the breeding of lilies was analyzed.",

keywords = "Intra-sectional hybrid, Meiosis, Pollen viability, SDR, Sexual polyploid",

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doi = "10.1270/jsbbs.54.13",

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volume = "54",

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journal = "Breeding Science",

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T1 - Occurrence of SDR 2N-gametes in Lilium hybrids

AU - Lim, Ki Byung

AU - Shen, Tsai Mu

AU - Barba-Gonzalez, Rodrigo

AU - Ramanna, M. S.

AU - Van Tuyl, Jaap M.

PY - 2004/3

Y1 - 2004/3

N2 - The mechanism of SDR 2n-pollen formation was analyzed in two intra-sectional diploid (2n = 2x = 24) Lilium hybrids (Enchantment x L. pumilum). Variable frequencies of 2n-pollen were found. Meiotic analysis indicated that the intra-sectional hybrids showed perfect chromosome pairing in most cases at metaphase I and normal anaphase I movement of pollen mother cells (PMCs), but produced 2n-pollen by second division restitution (SDR). A high bivalent formation (11.9II and 11.8II, respectively) at metaphase I, irregular meiotic division such as unbalanced chromosome separation and chromatic fragmentation resulted yet in acceptable pollen fertility for cross-pollination. The hybrids were fertile, and when used as male parents, offspring could be generated. The significance of the occurrence of 2n-pollen for the breeding of lilies was analyzed.

AB - The mechanism of SDR 2n-pollen formation was analyzed in two intra-sectional diploid (2n = 2x = 24) Lilium hybrids (Enchantment x L. pumilum). Variable frequencies of 2n-pollen were found. Meiotic analysis indicated that the intra-sectional hybrids showed perfect chromosome pairing in most cases at metaphase I and normal anaphase I movement of pollen mother cells (PMCs), but produced 2n-pollen by second division restitution (SDR). A high bivalent formation (11.9II and 11.8II, respectively) at metaphase I, irregular meiotic division such as unbalanced chromosome separation and chromatic fragmentation resulted yet in acceptable pollen fertility for cross-pollination. The hybrids were fertile, and when used as male parents, offspring could be generated. The significance of the occurrence of 2n-pollen for the breeding of lilies was analyzed.

KW - Intra-sectional hybrid

KW - Meiosis

KW - Pollen viability

KW - SDR

KW - Sexual polyploid

UR - https://www.scopus.com/pages/publications/1842540319

U2 - 10.1270/jsbbs.54.13

DO - 10.1270/jsbbs.54.13

M3 - Article

AN - SCOPUS:1842540319

SN - 1344-7610

VL - 54

SP - 13

EP - 18

JO - Breeding Science

JF - Breeding Science

IS - 1

ER -

Occurrence of SDR 2N-gametes in Lilium hybrids

Abstract

Keywords

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