OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs)

Kwang Won Seo; Sae Rom Lee; Dilli Ram Bhandari; Kyoung Hwan Roh; Sang Bum Park; Ah Young So; Ji Won Jung; Min Soo Seo; Soo Kyung Kang; Yong Soon Lee; Kyung Sun Kang

doi:10.1016/j.bbrc.2009.04.094

OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs)

Kwang Won Seo, Sae Rom Lee, Dilli Ram Bhandari, Kyoung Hwan Roh, Sang Bum Park, Ah Young So, Ji Won Jung, Min Soo Seo, Soo Kyung Kang, Yong Soon Lee, Kyung Sun Kang

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

53 Scopus citations

Abstract

The OCT4A gene, a POU homeodomain transcription factor, has been shown to be expressed in embryonic stem cells (ESC) as well as hUCB-MSCs. In this study, the roles played by OCT4A in hUCB-MSCs were determined by stably inhibiting OCT4A with lenti-viral vector-based small hairpin RNA (shRNA). A decreased rate of cell proliferation was observed in OCT4-inhibited hUCB-MSCs. Down-regulation of CCNA2 expression in OCT4-inhibited hUCB-MSCs was confirmed by RT-PCR and real-time RT-PCR analysis in three genetically independent hUCB-MSC clones. Adipogenic differentiation was also suppressed in OCT4-inhibited hUCB-MSCs. The up-regulation of DTX1 and down-regulation of HDAC1, 2, and 4 expressions may be related to this differentiation deformity. The expression of other transcription factors, including SOX2, REX1 and c-MYC, was also affected by OCT4 inhibition in hUCB-MSCs. In conclusion, these finding suggest that OCT4A performs functionally conserved roles in hUCB-MSCs, making its expression biologically important for ex vivo culture of hUCB-MSCs.

Original language	English
Pages (from-to)	120-125
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	384
Issue number	1
DOIs	https://doi.org/10.1016/j.bbrc.2009.04.094
State	Published - 19 Jun 2009

Keywords

Multi-potency
OCT4
shRNA
Stemness
Umbilical cord blood-derived mesenchymal stem cells

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Seo, K. W., Lee, S. R., Bhandari, D. R., Roh, K. H., Park, S. B., So, A. Y., Jung, J. W., Seo, M. S., Kang, S. K., Lee, Y. S., & Kang, K. S. (2009). OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs). Biochemical and Biophysical Research Communications, 384(1), 120-125. https://doi.org/10.1016/j.bbrc.2009.04.094

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title = "OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs)",

abstract = "The OCT4A gene, a POU homeodomain transcription factor, has been shown to be expressed in embryonic stem cells (ESC) as well as hUCB-MSCs. In this study, the roles played by OCT4A in hUCB-MSCs were determined by stably inhibiting OCT4A with lenti-viral vector-based small hairpin RNA (shRNA). A decreased rate of cell proliferation was observed in OCT4-inhibited hUCB-MSCs. Down-regulation of CCNA2 expression in OCT4-inhibited hUCB-MSCs was confirmed by RT-PCR and real-time RT-PCR analysis in three genetically independent hUCB-MSC clones. Adipogenic differentiation was also suppressed in OCT4-inhibited hUCB-MSCs. The up-regulation of DTX1 and down-regulation of HDAC1, 2, and 4 expressions may be related to this differentiation deformity. The expression of other transcription factors, including SOX2, REX1 and c-MYC, was also affected by OCT4 inhibition in hUCB-MSCs. In conclusion, these finding suggest that OCT4A performs functionally conserved roles in hUCB-MSCs, making its expression biologically important for ex vivo culture of hUCB-MSCs.",

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AU - Bhandari, Dilli Ram

AU - Roh, Kyoung Hwan

AU - Park, Sang Bum

AU - So, Ah Young

AU - Jung, Ji Won

AU - Seo, Min Soo

AU - Kang, Soo Kyung

AU - Lee, Yong Soon

AU - Kang, Kyung Sun

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OCT4A contributes to the stemness and multi-potency of human umbilical cord blood-derived multipotent stem cells (hUCB-MSCs)

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Keywords

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