One-step multiplex reverse transcription-polymerase chain reaction for the simultaneous detection of three rice viruses

Sang Yun Cho, Rae Dong Jeong, Young Nam Yoon, Su Heon Lee, Dong Bum Shin, Hang Won Kang, Bong Choon Lee

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

Rice stripe virus (RSV), Rice black-streaked dwarf virus (RBSDV), and Rice dwarf virus (RDV) are major rice-infecting viruses in Korea that can cause serious crop losses. A one-step multiplex reverse transcription-polymerase chain reaction (mRT-PCR) was developed for the simultaneous detection of these rice viruses. Three sets of specific primers targeted to the capsid protein coding genes of RSV, RBSDV, and RDV were used to amplify fragments that were 703. bp, 485. bp, and 252. bp, respectively. The one-step mRT-PCR assay proved to be a sensitive and rapid method for detecting the three rice viruses. This method could be used to facilitate better control of rice viruses.

Original languageEnglish
Pages (from-to)674-678
Number of pages5
JournalJournal of Virological Methods
Volume193
Issue number2
DOIs
StatePublished - Nov 2013

Keywords

  • One-step multiplex RT-PCR
  • Rice black-streaked dwarf virus
  • Rice dwarf virus
  • Rice stripe virus
  • Simultaneous detection

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