Optical clearing assisted confocal microscopy of ex vivo transgenic mouse skin

Eunjoo Song; Yoonjoon Ahn; Jinhyo Ahn; Soyeon Ahn; Changhwan Kim; Sanghoon Choi; Richard Martin Boutilier; Yongjoong Lee; Pilhan Kim; Ho Lee

doi:10.1016/j.optlastec.2015.03.020

Optical clearing assisted confocal microscopy of ex vivo transgenic mouse skin

Eunjoo Song, Yoonjoon Ahn, Jinhyo Ahn, Soyeon Ahn, Changhwan Kim, Sanghoon Choi, Richard Martin Boutilier, Yongjoong Lee, Pilhan Kim, Ho Lee

School of Mechanical Engineering

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

We examined the optical clearing assisted confocal microscopy of the transgenic mouse skin. The pinna and dorsal skin were imaged with a confocal microscope after the application of glycerol and FocusClear. In case of the glycerol-treated pinna, the clearing was minimal due to the inefficient permeability. However, the imaging depth was improved when the pinna was treated with FocusClear. In case of dorsal skin, we were able to image deeply to the subcutaneous connective tissue with both agents. Various skin structures such as the vessel, epithelium cells, cartilage, dermal cells, and hair follicles were clearly imaged.

Original language	English
Pages (from-to)	69-76
Number of pages	8
Journal	Optics and Laser Technology
Volume	73
DOIs	https://doi.org/10.1016/j.optlastec.2015.03.020
State	Published - 10 May 2015

Keywords

Optical clearing
Tissue clearing
Transgenic mouse skin

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10.1016/j.optlastec.2015.03.020

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title = "Optical clearing assisted confocal microscopy of ex vivo transgenic mouse skin",

abstract = "We examined the optical clearing assisted confocal microscopy of the transgenic mouse skin. The pinna and dorsal skin were imaged with a confocal microscope after the application of glycerol and FocusClear. In case of the glycerol-treated pinna, the clearing was minimal due to the inefficient permeability. However, the imaging depth was improved when the pinna was treated with FocusClear. In case of dorsal skin, we were able to image deeply to the subcutaneous connective tissue with both agents. Various skin structures such as the vessel, epithelium cells, cartilage, dermal cells, and hair follicles were clearly imaged.",

keywords = "Optical clearing, Tissue clearing, Transgenic mouse skin",

author = "Eunjoo Song and Yoonjoon Ahn and Jinhyo Ahn and Soyeon Ahn and Changhwan Kim and Sanghoon Choi and Boutilier, \{Richard Martin\} and Yongjoong Lee and Pilhan Kim and Ho Lee",

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T1 - Optical clearing assisted confocal microscopy of ex vivo transgenic mouse skin

AU - Song, Eunjoo

AU - Ahn, Yoonjoon

AU - Ahn, Jinhyo

AU - Ahn, Soyeon

AU - Kim, Changhwan

AU - Choi, Sanghoon

AU - Boutilier, Richard Martin

AU - Lee, Yongjoong

AU - Kim, Pilhan

AU - Lee, Ho

PY - 2015/5/10

Y1 - 2015/5/10

N2 - We examined the optical clearing assisted confocal microscopy of the transgenic mouse skin. The pinna and dorsal skin were imaged with a confocal microscope after the application of glycerol and FocusClear. In case of the glycerol-treated pinna, the clearing was minimal due to the inefficient permeability. However, the imaging depth was improved when the pinna was treated with FocusClear. In case of dorsal skin, we were able to image deeply to the subcutaneous connective tissue with both agents. Various skin structures such as the vessel, epithelium cells, cartilage, dermal cells, and hair follicles were clearly imaged.

AB - We examined the optical clearing assisted confocal microscopy of the transgenic mouse skin. The pinna and dorsal skin were imaged with a confocal microscope after the application of glycerol and FocusClear. In case of the glycerol-treated pinna, the clearing was minimal due to the inefficient permeability. However, the imaging depth was improved when the pinna was treated with FocusClear. In case of dorsal skin, we were able to image deeply to the subcutaneous connective tissue with both agents. Various skin structures such as the vessel, epithelium cells, cartilage, dermal cells, and hair follicles were clearly imaged.

KW - Optical clearing

KW - Tissue clearing

KW - Transgenic mouse skin

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DO - 10.1016/j.optlastec.2015.03.020

M3 - Article

AN - SCOPUS:84929070101

SN - 0030-3992

VL - 73

SP - 69

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JO - Optics and Laser Technology

JF - Optics and Laser Technology

ER -

Optical clearing assisted confocal microscopy of ex vivo transgenic mouse skin

Abstract

Keywords

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