TY - JOUR
T1 - Optical resolution of racemic α-amino acids on a dynamic chiral stationary phase derived from (S)-leucinol by ligand exchange chromatography
AU - Hyun, Myung Ho
AU - Ryoo, Jae Jeong
PY - 1996
Y1 - 1996
N2 - A dynamic chiral stationary phase (CSP 7) for resolving racemic α-amino acids have been prepared by hydrophobically loading (S)-N,N-carboxymethyl dodecyl leucinol monosodium salt onto a commercial reverse phase octadecyl silica gel column. CSP 7 was successfully employed in resolving various racemic α-amino acids. The chromatographic results for resolving various racemic α-amino acids on CSP 7 have been found to be generally better as expected from the chiral recognition model proposed than those on the previously reported dynamic CSP (2), (R)-N,N-carboxymethyl dodecyl alaninol monosodium salt which is hydrophobically bound onto a commercial reverse phase octadecyl silica gel column. Especially, CSP-7 seems to be more attractive than CSP 2 in that CSP 7 shows reasonably good resolving ability for the broad range of racemic α-amino acids at the high content of organic modifier (20% CH3CN) in the aqueous mobile phase while CSP 2 shows very poor resolving ability.
AB - A dynamic chiral stationary phase (CSP 7) for resolving racemic α-amino acids have been prepared by hydrophobically loading (S)-N,N-carboxymethyl dodecyl leucinol monosodium salt onto a commercial reverse phase octadecyl silica gel column. CSP 7 was successfully employed in resolving various racemic α-amino acids. The chromatographic results for resolving various racemic α-amino acids on CSP 7 have been found to be generally better as expected from the chiral recognition model proposed than those on the previously reported dynamic CSP (2), (R)-N,N-carboxymethyl dodecyl alaninol monosodium salt which is hydrophobically bound onto a commercial reverse phase octadecyl silica gel column. Especially, CSP-7 seems to be more attractive than CSP 2 in that CSP 7 shows reasonably good resolving ability for the broad range of racemic α-amino acids at the high content of organic modifier (20% CH3CN) in the aqueous mobile phase while CSP 2 shows very poor resolving ability.
UR - http://www.scopus.com/inward/record.url?scp=0029823955&partnerID=8YFLogxK
U2 - 10.1080/10826079608014044
DO - 10.1080/10826079608014044
M3 - Article
AN - SCOPUS:0029823955
SN - 1082-6076
VL - 19
SP - 2635
EP - 2648
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 16
ER -