TY - JOUR
T1 - Optimization and application of a dithiobis-succinimidyl propionate-modified immunosensor platform to detect Listeria monocytogenes in chicken skin
AU - Park, Mi Kyung
AU - Park, Jang Won
AU - Oh, Jun Hyun
PY - 2012/8
Y1 - 2012/8
N2 - This research aims to demonstrate the potential feasibility of a surface modified gold-coated immunosensor platform combined with light microscopic imaging system (LMIS) to detect Listeria monocytogenes in chicken. The reactivity of custom prepared antibodies (P-pAbs) was significantly higher than the reactivity of commercial antibodies and the P-pAbs were highly specific with L. monocytogenes strains. A gold-coated sensor platform modified with dithiobis-succinimidyl propionate (DSP) exhibited a 40% increase in binding efficiency, compared to the gold-coated sensor platform. The optimum concentration and incubation time for P-pAbs immobilization on DSP-modified sensor platform was determined as 100 μg/ml and 45 min, respectively. The optimum incubation time and pH range for binding between L. monocytogenes and P-pAbs were determined as 45 min and pH 5.5-9.5, respectively. The limit of detection of DSP-modified immunosensor platform to detect L. monocytogenes in chicken skin was determined as 10 3 CFU/25 g, exhibiting 66% positive rate. Observing the images from LMIS demonstrated that the DSP-modified immunosensor platform provided an easy differentiation between L. monocytogenes and attached food particles in chicken products.
AB - This research aims to demonstrate the potential feasibility of a surface modified gold-coated immunosensor platform combined with light microscopic imaging system (LMIS) to detect Listeria monocytogenes in chicken. The reactivity of custom prepared antibodies (P-pAbs) was significantly higher than the reactivity of commercial antibodies and the P-pAbs were highly specific with L. monocytogenes strains. A gold-coated sensor platform modified with dithiobis-succinimidyl propionate (DSP) exhibited a 40% increase in binding efficiency, compared to the gold-coated sensor platform. The optimum concentration and incubation time for P-pAbs immobilization on DSP-modified sensor platform was determined as 100 μg/ml and 45 min, respectively. The optimum incubation time and pH range for binding between L. monocytogenes and P-pAbs were determined as 45 min and pH 5.5-9.5, respectively. The limit of detection of DSP-modified immunosensor platform to detect L. monocytogenes in chicken skin was determined as 10 3 CFU/25 g, exhibiting 66% positive rate. Observing the images from LMIS demonstrated that the DSP-modified immunosensor platform provided an easy differentiation between L. monocytogenes and attached food particles in chicken products.
KW - Chicken
KW - Dithiobis-succinimidyl propionate (DSP)
KW - Immunosensor platform
KW - Listeria monocytogenes
KW - Polyclonal antibodies
UR - http://www.scopus.com/inward/record.url?scp=84864287318&partnerID=8YFLogxK
U2 - 10.1016/j.snb.2012.04.017
DO - 10.1016/j.snb.2012.04.017
M3 - Article
AN - SCOPUS:84864287318
SN - 0925-4005
VL - 171-172
SP - 323
EP - 331
JO - Sensors and Actuators, B: Chemical
JF - Sensors and Actuators, B: Chemical
ER -