Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

Aung Htay Naing; Kyeung Il Park; Mi Young Chung; Ki Byung Lim; Chang Kil Kim

doi:10.1007/s40415-015-0143-0

Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

Aung Htay Naing, Kyeung Il Park, Mi Young Chung, Ki Byung Lim, Chang Kil Kim

Research output: Contribution to journal › Article › peer-review

27 Scopus citations

Abstract

Factors affecting shoot regeneration were optimized in order to develop an efficient protocol for shoot regeneration in leaf explants of the chrysanthemum (Chrysanthemum morifolium Ramat) cultivar Shinma. The maximum number of shoots per explant (9.7) was regenerated in leaf explants of 6-week-old donor plants cultured on Murashige–Skoog medium containing a combination of 0.5 mg L⁻¹ 6-benzyladenine and 0.5 mg L⁻¹ α-naphthaleneacetic acid with initial dark treatment for 7 days. Among the different auxins tested, indolebutyric acid was the most effective for root induction and development, whereas the presence of ethylene inhibitors strongly suppressed shoot regeneration. Ploidy levels were analyzed by flow cytometry: there was no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The new protocol will facilitate genetic transformation and micropropagation of chrysanthemum cv. Shinma.

Original language	English
Pages (from-to)	975-984
Number of pages	10
Journal	Revista Brasileira de Botanica
Volume	39
Issue number	4
DOIs	https://doi.org/10.1007/s40415-015-0143-0
State	Published - 1 Dec 2016

Keywords

Dark treatment
Donor plant age
Ethylene inhibitors
Flow cytometry
Ornamental plant
Plant growth regulator

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10.1007/s40415-015-0143-0

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title = "Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma",

abstract = "Factors affecting shoot regeneration were optimized in order to develop an efficient protocol for shoot regeneration in leaf explants of the chrysanthemum (Chrysanthemum morifolium Ramat) cultivar Shinma. The maximum number of shoots per explant (9.7) was regenerated in leaf explants of 6-week-old donor plants cultured on Murashige–Skoog medium containing a combination of 0.5 mg L−1 6-benzyladenine and 0.5 mg L−1 α-naphthaleneacetic acid with initial dark treatment for 7 days. Among the different auxins tested, indolebutyric acid was the most effective for root induction and development, whereas the presence of ethylene inhibitors strongly suppressed shoot regeneration. Ploidy levels were analyzed by flow cytometry: there was no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The new protocol will facilitate genetic transformation and micropropagation of chrysanthemum cv. Shinma.",

keywords = "Dark treatment, Donor plant age, Ethylene inhibitors, Flow cytometry, Ornamental plant, Plant growth regulator",

author = "Naing, \{Aung Htay\} and \{Il Park\}, Kyeung and Chung, \{Mi Young\} and Lim, \{Ki Byung\} and Kim, \{Chang Kil\}",

note = "Publisher Copyright: {\textcopyright} 2015, Botanical Society of Sao Paulo.",

year = "2016",

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doi = "10.1007/s40415-015-0143-0",

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T1 - Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

AU - Naing, Aung Htay

AU - Il Park, Kyeung

AU - Chung, Mi Young

AU - Lim, Ki Byung

AU - Kim, Chang Kil

PY - 2016/12/1

Y1 - 2016/12/1

N2 - Factors affecting shoot regeneration were optimized in order to develop an efficient protocol for shoot regeneration in leaf explants of the chrysanthemum (Chrysanthemum morifolium Ramat) cultivar Shinma. The maximum number of shoots per explant (9.7) was regenerated in leaf explants of 6-week-old donor plants cultured on Murashige–Skoog medium containing a combination of 0.5 mg L−1 6-benzyladenine and 0.5 mg L−1 α-naphthaleneacetic acid with initial dark treatment for 7 days. Among the different auxins tested, indolebutyric acid was the most effective for root induction and development, whereas the presence of ethylene inhibitors strongly suppressed shoot regeneration. Ploidy levels were analyzed by flow cytometry: there was no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The new protocol will facilitate genetic transformation and micropropagation of chrysanthemum cv. Shinma.

AB - Factors affecting shoot regeneration were optimized in order to develop an efficient protocol for shoot regeneration in leaf explants of the chrysanthemum (Chrysanthemum morifolium Ramat) cultivar Shinma. The maximum number of shoots per explant (9.7) was regenerated in leaf explants of 6-week-old donor plants cultured on Murashige–Skoog medium containing a combination of 0.5 mg L−1 6-benzyladenine and 0.5 mg L−1 α-naphthaleneacetic acid with initial dark treatment for 7 days. Among the different auxins tested, indolebutyric acid was the most effective for root induction and development, whereas the presence of ethylene inhibitors strongly suppressed shoot regeneration. Ploidy levels were analyzed by flow cytometry: there was no ploidy variation between the regenerated plants and the mother plant grown under greenhouse conditions. The new protocol will facilitate genetic transformation and micropropagation of chrysanthemum cv. Shinma.

KW - Dark treatment

KW - Donor plant age

KW - Ethylene inhibitors

KW - Flow cytometry

KW - Ornamental plant

KW - Plant growth regulator

UR - https://www.scopus.com/pages/publications/85006401193

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DO - 10.1007/s40415-015-0143-0

M3 - Article

AN - SCOPUS:85006401193

SN - 0100-8404

VL - 39

SP - 975

EP - 984

JO - Revista Brasileira de Botanica

JF - Revista Brasileira de Botanica

IS - 4

ER -

Optimization of factors affecting efficient shoot regeneration in chrysanthemum cv. Shinma

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Keywords

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