Optimization of key process variables for enhanced hydrogen production by Enterobacter aerogenes using statistical methods

Ji Hye Jo, Dae Sung Lee, Donghee Park, Woo Seok Choe, Jong Moon Park

Research output: Contribution to journalArticlepeer-review

141 Scopus citations

Abstract

The individual and mutual effects of glucose concentration, temperature and pH on the hydrogen production by Enterobacter aerogenes were investigated in a batch system. A Box-Behnken design and response surface methodology (RSM) were employed to determine the optimum condition for enhanced hydrogen production. The hydrogen production rate was investigated by simultaneously changing the three independent variables, which all had significant influences on the hydrogen production rate. The maximum hydrogen production rate of 425.8 ml H2 (g dry cell h)-1 was obtained under the optimum condition of glucose concentration 118.06 mM, temperature 38 °C and pH 6.13. The experimental results showed that the RSM with the Box-Behnken design was a useful tool for achieving high rate of hydrogen production by E. aerogenes.

Original languageEnglish
Pages (from-to)2061-2066
Number of pages6
JournalBioresource Technology
Volume99
Issue number6
DOIs
StatePublished - Apr 2008

Keywords

  • Enterobacter aerogenes
  • Hydrogen production rate
  • Response surface methodology (RSM)

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