Osteogenesis of adipose-derived and bone marrow stem cells with polycaprolactone/tricalcium phosphate and three-dimensional printing technology in a dog model of maxillary bone defects

Jeong Woo Lee; Seung Gyun Chu; Hak Tae Kim; Kang Young Choi; Eun Jung Oh; Jin Hyung Shim; Won Soo Yun; Jung Bo Huh; Sung Hwan Moon; Seong Soo Kang; Ho Yun Chung

doi:10.3390/polym9090450

Osteogenesis of adipose-derived and bone marrow stem cells with polycaprolactone/tricalcium phosphate and three-dimensional printing technology in a dog model of maxillary bone defects

Jeong Woo Lee, Seung Gyun Chu, Hak Tae Kim, Kang Young Choi, Eun Jung Oh, Jin Hyung Shim, Won Soo Yun, Jung Bo Huh, Sung Hwan Moon, Seong Soo Kang, Ho Yun Chung

Department of Medicine

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Bone graft material should possess sufficient porosity and permeability to allow integration with native tissue and vascular invasion, and must satisfy oxygen and nutrient transport demands. In this study, we have examined the use of three-dimensional (3D)-printed polycaprolactone/tricalcium phosphate (PCL/TCP) composite material in bone grafting, to estimate the scope of its potential application in bone surgery. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) are known to enhance osteointegration. We hypothesized that a patient-specific 3D-printed solid scaffold could help preserve seeded ADSCs and BMSCs and enhance osteointegration. Diffuse osteogenic tissue formation was observed by micro-computed tomography with both stem cell types, and the ADSC group displayed similar osteogenesis compared to the BMSC group. In histological assessment, the scaffold pores showed abundant ossification in both groups. Reverse transcription polymerase chain reaction (RT-PCR) showed that the BMSC group had higher expression of genes associated with ossification, and this was confirmed byWestern blot analysis. The ADSC- and BMSC-seeded 3D-printed PCL/TCP scaffolds displayed promising enhancement of osteogenesis in a dog model of maxillary bone defects.

Original language	English
Article number	450
Journal	Polymers
Volume	9
Issue number	9
DOIs	https://doi.org/10.3390/polym9090450
State	Published - 15 Sep 2017

Keywords

3D-printed scaffold
Adipose-derived stem cells
Bone marrow stem cells
Dog
Maxillary bone
Osteogenesis
Polycaprolactone
Tricalcium phosphate

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Lee, J. W., Chu, S. G., Kim, H. T., Choi, K. Y., Oh, E. J., Shim, J. H., Yun, W. S., Huh, J. B., Moon, S. H., Kang, S. S., & Chung, H. Y. (2017). Osteogenesis of adipose-derived and bone marrow stem cells with polycaprolactone/tricalcium phosphate and three-dimensional printing technology in a dog model of maxillary bone defects. Polymers, 9(9), Article 450. https://doi.org/10.3390/polym9090450

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title = "Osteogenesis of adipose-derived and bone marrow stem cells with polycaprolactone/tricalcium phosphate and three-dimensional printing technology in a dog model of maxillary bone defects",

abstract = "Bone graft material should possess sufficient porosity and permeability to allow integration with native tissue and vascular invasion, and must satisfy oxygen and nutrient transport demands. In this study, we have examined the use of three-dimensional (3D)-printed polycaprolactone/tricalcium phosphate (PCL/TCP) composite material in bone grafting, to estimate the scope of its potential application in bone surgery. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) are known to enhance osteointegration. We hypothesized that a patient-specific 3D-printed solid scaffold could help preserve seeded ADSCs and BMSCs and enhance osteointegration. Diffuse osteogenic tissue formation was observed by micro-computed tomography with both stem cell types, and the ADSC group displayed similar osteogenesis compared to the BMSC group. In histological assessment, the scaffold pores showed abundant ossification in both groups. Reverse transcription polymerase chain reaction (RT-PCR) showed that the BMSC group had higher expression of genes associated with ossification, and this was confirmed byWestern blot analysis. The ADSC- and BMSC-seeded 3D-printed PCL/TCP scaffolds displayed promising enhancement of osteogenesis in a dog model of maxillary bone defects.",

keywords = "3D-printed scaffold, Adipose-derived stem cells, Bone marrow stem cells, Dog, Maxillary bone, Osteogenesis, Polycaprolactone, Tricalcium phosphate",

author = "Lee, {Jeong Woo} and Chu, {Seung Gyun} and Kim, {Hak Tae} and Choi, {Kang Young} and Oh, {Eun Jung} and Shim, {Jin Hyung} and Yun, {Won Soo} and Huh, {Jung Bo} and Moon, {Sung Hwan} and Kang, {Seong Soo} and Chung, {Ho Yun}",

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AU - Kim, Hak Tae

AU - Choi, Kang Young

AU - Oh, Eun Jung

AU - Shim, Jin Hyung

AU - Yun, Won Soo

AU - Huh, Jung Bo

AU - Moon, Sung Hwan

AU - Kang, Seong Soo

AU - Chung, Ho Yun

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AB - Bone graft material should possess sufficient porosity and permeability to allow integration with native tissue and vascular invasion, and must satisfy oxygen and nutrient transport demands. In this study, we have examined the use of three-dimensional (3D)-printed polycaprolactone/tricalcium phosphate (PCL/TCP) composite material in bone grafting, to estimate the scope of its potential application in bone surgery. Adipose-derived stem cells (ADSCs) and bone marrow stem cells (BMSCs) are known to enhance osteointegration. We hypothesized that a patient-specific 3D-printed solid scaffold could help preserve seeded ADSCs and BMSCs and enhance osteointegration. Diffuse osteogenic tissue formation was observed by micro-computed tomography with both stem cell types, and the ADSC group displayed similar osteogenesis compared to the BMSC group. In histological assessment, the scaffold pores showed abundant ossification in both groups. Reverse transcription polymerase chain reaction (RT-PCR) showed that the BMSC group had higher expression of genes associated with ossification, and this was confirmed byWestern blot analysis. The ADSC- and BMSC-seeded 3D-printed PCL/TCP scaffolds displayed promising enhancement of osteogenesis in a dog model of maxillary bone defects.

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Osteogenesis of adipose-derived and bone marrow stem cells with polycaprolactone/tricalcium phosphate and three-dimensional printing technology in a dog model of maxillary bone defects

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