Osteogenic activity of chitosan-based hybrid scaffold prepared by polyelectrolyte complex formation with alginate

Seung Hwan Lee; Ho Yun Chung; Hong In Shin; Dong Joon Park; Jin Hyun Choi

doi:10.1007/s13770-013-1114-9

Osteogenic activity of chitosan-based hybrid scaffold prepared by polyelectrolyte complex formation with alginate

Seung Hwan Lee, Ho Yun Chung, Hong In Shin, Dong Joon Park, Jin Hyun Choi

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

A chitosan (CS)/alginate (AG) hybrid scaffold was prepared through solid state polyelectrolyte complex (PEC) formation. The PEC formation was confirmed by Fourier-transformed infrared spectroscopy. Positive von Kossa staining of calcium depositions appeared in a large area of the CS/AG PEC scaffold cultured with osteoblastlike KUSA-A1 cells. The alkaline phosphatase activity of the cells cultured in the CS/AG scaffold was higher than that of the cells in the CS/hydroxyapatite (HA) scaffold. The osteogenic activity of the CS/AG PEC scaffold was considered comparable to that of the CS/HA scaffold. These findings suggest that modification of CS-based scaffold by PEC formation have a significant effect on the cell behavior towards osteogenic differentiation and bone tissue regeneration.

Original language	English
Pages (from-to)	106-112
Number of pages	7
Journal	Tissue Engineering and Regenerative Medicine
Volume	11
Issue number	2
DOIs	https://doi.org/10.1007/s13770-013-1114-9
State	Published - Apr 2014

Keywords

alginate
chitosan
osteogenic activity
polyelectrolyte complex
scaffold

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10.1007/s13770-013-1114-9

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abstract = "A chitosan (CS)/alginate (AG) hybrid scaffold was prepared through solid state polyelectrolyte complex (PEC) formation. The PEC formation was confirmed by Fourier-transformed infrared spectroscopy. Positive von Kossa staining of calcium depositions appeared in a large area of the CS/AG PEC scaffold cultured with osteoblastlike KUSA-A1 cells. The alkaline phosphatase activity of the cells cultured in the CS/AG scaffold was higher than that of the cells in the CS/hydroxyapatite (HA) scaffold. The osteogenic activity of the CS/AG PEC scaffold was considered comparable to that of the CS/HA scaffold. These findings suggest that modification of CS-based scaffold by PEC formation have a significant effect on the cell behavior towards osteogenic differentiation and bone tissue regeneration.",

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T1 - Osteogenic activity of chitosan-based hybrid scaffold prepared by polyelectrolyte complex formation with alginate

AU - Lee, Seung Hwan

AU - Chung, Ho Yun

AU - Shin, Hong In

AU - Park, Dong Joon

AU - Choi, Jin Hyun

PY - 2014/4

Y1 - 2014/4

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AB - A chitosan (CS)/alginate (AG) hybrid scaffold was prepared through solid state polyelectrolyte complex (PEC) formation. The PEC formation was confirmed by Fourier-transformed infrared spectroscopy. Positive von Kossa staining of calcium depositions appeared in a large area of the CS/AG PEC scaffold cultured with osteoblastlike KUSA-A1 cells. The alkaline phosphatase activity of the cells cultured in the CS/AG scaffold was higher than that of the cells in the CS/hydroxyapatite (HA) scaffold. The osteogenic activity of the CS/AG PEC scaffold was considered comparable to that of the CS/HA scaffold. These findings suggest that modification of CS-based scaffold by PEC formation have a significant effect on the cell behavior towards osteogenic differentiation and bone tissue regeneration.

KW - alginate

KW - chitosan

KW - osteogenic activity

KW - polyelectrolyte complex

KW - scaffold

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ER -

Osteogenic activity of chitosan-based hybrid scaffold prepared by polyelectrolyte complex formation with alginate

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