Overproduction of α-glucosidase in Aspergillus niger transformed with the cloned gene aglA

Dong Gun Lee, Ikuko Nishimura-Masuda, Akira Nakamura, Makoto Hidaka, Haruhiko Masaki, Takeshi Uozumi

Research output: Contribution to journalArticlepeer-review

10 Scopus citations

Abstract

We have transformed an industrial strain, Aspergillus niger GN-3, with the α-glucosidase gene (aglA) from the same strain. Southern hybridization analysis revealed that transformants had multiple copies of the cloned DNA inserted into the host genome. An 11-fold improvement of enzyme production was achieved by transformation with a DNA fragment composed of 1.11 kb of the 5' noncoding region, 3.12 kb of the coding region containing three introns, and 1.2 kb of the 3' noncoding region. It was found that the 3' noncoding region (1.2 kb) was preferable for maximum production of the enzyme in the transformant.

Original languageEnglish
Pages (from-to)177-181
Number of pages5
JournalJournal of General and Applied Microbiology
Volume44
Issue number3
DOIs
StatePublished - 1998

Keywords

  • α-glucosidase
  • aglA
  • Aspergillus niger
  • Overproduction
  • Transformation

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