Overproduction of sweet potato peroxidases in transgenic tobacco plants

Gyung Hye Huh; Byung Wook Yun; Haeng Soon Lee; Jin Ki Jo; Sang Soo Kwak

doi:10.1016/S0031-9422(97)00602-X

Overproduction of sweet potato peroxidases in transgenic tobacco plants

Gyung Hye Huh, Byung Wook Yun, Haeng Soon Lee, Jin Ki Jo, Sang Soo Kwak

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

Two peroxidase (POD) cDNAs, anionic (swpa1) and neutral (swpn1) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimetic gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants Carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants.

Original language	English
Pages (from-to)	695-700
Number of pages	6
Journal	Phytochemistry
Volume	47
Issue number	5
DOIs	https://doi.org/10.1016/S0031-9422(97)00602-X
State	Published - Mar 1998

Keywords

Convolvulaceae
Ipomoea batatas
Nicotiana tabacum
Peroxidase
Solanaceae
Superoxide dismutase
Trans-genic plants

Access to Document

10.1016/S0031-9422(97)00602-X

Cite this

@article{01af644761fc4a609edde2abc16a70f7,

title = "Overproduction of sweet potato peroxidases in transgenic tobacco plants",

abstract = "Two peroxidase (POD) cDNAs, anionic (swpa1) and neutral (swpn1) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimetic gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants Carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants.",

keywords = "Convolvulaceae, Ipomoea batatas, Nicotiana tabacum, Peroxidase, Solanaceae, Superoxide dismutase, Trans-genic plants",

author = "Huh, {Gyung Hye} and Yun, {Byung Wook} and Lee, {Haeng Soon} and Jo, {Jin Ki} and Kwak, {Sang Soo}",

year = "1998",

month = mar,

doi = "10.1016/S0031-9422(97)00602-X",

language = "English",

volume = "47",

pages = "695--700",

journal = "Phytochemistry",

issn = "0031-9422",

publisher = "Elsevier Ltd.",

number = "5",

}

TY - JOUR

T1 - Overproduction of sweet potato peroxidases in transgenic tobacco plants

AU - Huh, Gyung Hye

AU - Yun, Byung Wook

AU - Lee, Haeng Soon

AU - Jo, Jin Ki

AU - Kwak, Sang Soo

PY - 1998/3

Y1 - 1998/3

N2 - Two peroxidase (POD) cDNAs, anionic (swpa1) and neutral (swpn1) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimetic gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants Carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants.

AB - Two peroxidase (POD) cDNAs, anionic (swpa1) and neutral (swpn1) POD derived from suspension cultures of sweet potato (Ipomoea batatas), were used to express high levels in transgenic plants. Two sweet potato PODs were overproduced in two cultivars of transformed tobacco (Nicotiana tabacum) plants, cv. Bel W3 and cv. Samsun by introducing a chimetic gene composed of the cauliflower mosaic virus 35S promoter and POD cDNA. Young fully expanded leaves of transgenic plants of both cultivars showed higher POD activity than those of nontransgenic plants. Bel W3 transgenic plants Carrying anionic POD had total POD activity ca five times higher than control plants. However, no significant differences were observed in superoxide dismutase activity between transgenic and nontransgenic plants. Transgenic Bel W3 and Samsun tobacco plants with either anionic or neutral POD isoenzyme did not show distinctive phenotypes compared with untransformed plants.

KW - Convolvulaceae

KW - Ipomoea batatas

KW - Nicotiana tabacum

KW - Peroxidase

KW - Solanaceae

KW - Superoxide dismutase

KW - Trans-genic plants

UR - http://www.scopus.com/inward/record.url?scp=0032030486&partnerID=8YFLogxK

U2 - 10.1016/S0031-9422(97)00602-X

DO - 10.1016/S0031-9422(97)00602-X

M3 - Article

AN - SCOPUS:0032030486

SN - 0031-9422

VL - 47

SP - 695

EP - 700

JO - Phytochemistry

JF - Phytochemistry

IS - 5

ER -

Overproduction of sweet potato peroxidases in transgenic tobacco plants

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this