Abstract
Background: Recent study has demonstrated that Sasa quelpaertensis (Korean name, Jeju-Joritdae) extracts inhibit cellular melanogenesis implicating potential use in the control of skin pigmentation. Objectives: The purpose of the present study was to elucidate the active constituents of this plant inhibiting melanogenesis and the associated mechanism. Methods: The effect of the plant-derived materials on melanin production and/or tyrosinase expression was examined in murine melanoma B16/F10 cells and neonatal human melanocytes. Results: When tested in melanoma B16/F10 cells treated with the α-melanocyte stimulating hormone (α-MSH), the aqueous ethanol extract of S. quelpaertensis culm inhibited the cellular melanogenesis more effectively than its leaf extract. A major active compound was isolated from the culm extract by solvent fractionation and column chromatography, and identified to be p-coumaric acid by spectroscopic and chromatographic analyses. The compound (p-coumaric acid) inhibited α-MSH-stimulated cellular melanogenesis more effectively than arbutin or other structurally similar compounds including 3-(4-hydroxyphenyl) propionic acid, cinnamic acid and caffeic acid. It also attenuated α-MSH-dependent increase of tyrosinase protein. The antimelanogenic effect of p-coumaric acid was also verified in neonatal human melanocytes. Conclusions: The present study identified p-coumaric acid as a main constituent of S. quelpaertensis inhibiting cellular melanogenesis. Because of its structural similarity, p-coumaric acid may interfere with l-tyrosine action in the control of tyrosinase expression in response to α-MSH.
Original language | English |
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Pages (from-to) | 292-299 |
Number of pages | 8 |
Journal | British Journal of Dermatology |
Volume | 159 |
Issue number | 2 |
DOIs | |
State | Published - Aug 2008 |
Keywords
- Bamboo
- Melanin
- Sasa quelpaertensis Nakai
- Tyrosinase
- p-coumaric acid