Particle image velocimetry of the blood flow in a micro-channel using the confocal laser scanning microscope

Wi Han Kim, Chan Il Kim, Sang Won Lee, Soo Hee Lim, Cheol Woo Park, Ho Lee, Min Kyu Park

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

We used video-rate Confocal Laser Scanning Microscopy (CLSM) to observe the motion of blood cells in a micro-channel. Video-rate CLSM allowed us to acquire images at the rate of 30 frames per second. The acquired images were used to perform Particle Image Velocimetry (PIV), thus providing the velocity profile of the blood in a micro-channel. While previous confocal microscopy-assisted PIV required exogenous micro/nano particles as the tracing particles, we employed blood cells as tracing particles for the CLSM in the reflection mode, which uses light back-scattered from the sample. The blood flow at various depths of the micro-channel was observed by adjusting the image plane of the microscope. The velocity profile at different depths of the channel was measured. The confocal micro-PIV technique used in the study was able to measure blood velocity up to a few hundreds μm/sec, equivalent to the blood velocity in the capillaries of a live animal. It is expected that the technique presented can be applied for in vivo blood flow measurement in the capillaries of live animals.

Original languageEnglish
Pages (from-to)42-48
Number of pages7
JournalJournal of the Optical Society of Korea
Volume14
Issue number1
DOIs
StatePublished - Mar 2010

Keywords

  • Blood flow
  • Confocal microscopy
  • Particle image velocimetry

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