Abstract
This study employing a rodent model of acute pain investigated the influence of carrageenan-induced inflammation on the ability of S-α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor activation to induce membrane currents and rises in cytosolic free calcium concentration ([Ca2+]i) in the rat substantia gelatinosa (SG) neurons using simultaneous whole-cell patch-clamp recording and fura-2 calcium imaging in spinal cord slices of L4-L5 segments. The novel finding of this study is that carrageenan-induced inflammation, in the presence of cyclothiazide, an inhibitor of AMPA receptor desensitization, produces a sustained facilitation of the AMPA-mediated membrane current and rises in [Ca2+]i in both the soma and proximal dendrites of SG neurons recorded on the injected side 3 h after the induction of inflammation. These results suggest that in carrageenan-inflamed rats AMPA receptors undergo some alterations that influence AMPA receptors desensitization and/or sensitivity to cyclothiazide.
Original language | English |
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Pages (from-to) | 461-469 |
Number of pages | 9 |
Journal | Cell Calcium |
Volume | 35 |
Issue number | 5 |
DOIs | |
State | Published - May 2004 |
Keywords
- AMPA
- Gelatinosa
- Inflammation
- Substantia