Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

Soomin Chae; Hyun Kyung Lee; Yoo Kyung Kim; Hyo Jung Sim; Yoorim Ji; Chowon Kim; Tayaba Ismail; Jeen Woo Park; Oh Shin Kwon; Beom Sik Kang; Dong Seok Lee; Jong Sup Bae; Sang Hyun Kim; Kyoung Jin Min; Taeg Kyu Kwon; Mae Ja Park; Jin Kwan Han; Taejoon Kwon; Tae Joo Park; Hyun Shik Lee

doi:10.1038/s41598-017-09262-6

Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

Soomin Chae
, Hyun Kyung Lee
, Yoo Kyung Kim
, Hyo Jung Sim
, Yoorim Ji
, Chowon Kim
, Tayaba Ismail
, Jeen Woo Park
, Oh Shin Kwon
, Beom Sik Kang
, Dong Seok Lee
, Jong Sup Bae
, Sang Hyun Kim
, Kyoung Jin Min
, Taeg Kyu Kwon
, Mae Ja Park
, Jin Kwan Han
, Taejoon Kwon
, Tae Joo Park
, Hyun Shik Lee

Kyungpook National University
Ulsan National Institute of Science and Technology
Keimyung University
Pohang University of Science and Technology

Research output: Contribution to journal › Article › peer-review

25 Scopus citations

Abstract

Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H₂O₂ and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H₂O₂) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.

Original language	English
Article number	8874
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-09262-6
State	Published - 1 Dec 2017

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SDG 3 Good Health and Well-being

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Chae, S., Lee, H. K., Kim, Y. K., Jung Sim, H., Ji, Y., Kim, C., Ismail, T., Park, J. W., Kwon, O. S., Kang, B. S., Lee, D. S., Bae, J. S., Kim, S. H., Min, K. J., Kyu Kwon, T., Park, M. J., Han, J. K., Kwon, T., Park, T. J., & Lee, H. S. (2017). Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels. Scientific Reports, 7(1), Article 8874. https://doi.org/10.1038/s41598-017-09262-6

@article{6cfca7936d6a44a29684edc41c742dc5,

title = "Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels",

abstract = "Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.",

author = "Soomin Chae and Lee, \{Hyun Kyung\} and Kim, \{Yoo Kyung\} and \{Jung Sim\}, Hyo and Yoorim Ji and Chowon Kim and Tayaba Ismail and Park, \{Jeen Woo\} and Kwon, \{Oh Shin\} and Kang, \{Beom Sik\} and Lee, \{Dong Seok\} and Bae, \{Jong Sup\} and Kim, \{Sang Hyun\} and Min, \{Kyoung Jin\} and \{Kyu Kwon\}, Taeg and Park, \{Mae Ja\} and Han, \{Jin Kwan\} and Taejoon Kwon and Park, \{Tae Joo\} and Lee, \{Hyun Shik\}",

note = "Publisher Copyright: {\textcopyright} 2017, The Author(s).",

year = "2017",

month = dec,

day = "1",

doi = "10.1038/s41598-017-09262-6",

language = "English",

volume = "7",

journal = "Scientific Reports",

issn = "2045-2322",

publisher = "Nature Research",

number = "1",

}

Chae, S, Lee, HK, Kim, YK, Jung Sim, H, Ji, Y, Kim, C, Ismail, T, Park, JW, Kwon, OS , Kang, BS , Lee, DS , Bae, JS , Kim, SH, Min, KJ, Kyu Kwon, T, Park, MJ, Han, JK, Kwon, T, Park, TJ & Lee, HS 2017, 'Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels', Scientific Reports, vol. 7, no. 1, 8874. https://doi.org/10.1038/s41598-017-09262-6

TY - JOUR

T1 - Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

AU - Chae, Soomin

AU - Lee, Hyun Kyung

AU - Kim, Yoo Kyung

AU - Jung Sim, Hyo

AU - Ji, Yoorim

AU - Kim, Chowon

AU - Ismail, Tayaba

AU - Park, Jeen Woo

AU - Kwon, Oh Shin

AU - Kang, Beom Sik

AU - Lee, Dong Seok

AU - Bae, Jong Sup

AU - Kim, Sang Hyun

AU - Min, Kyoung Jin

AU - Kyu Kwon, Taeg

AU - Park, Mae Ja

AU - Han, Jin Kwan

AU - Kwon, Taejoon

AU - Park, Tae Joo

AU - Lee, Hyun Shik

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.

AB - Peroxiredoxin1 (Prdx1) is an antioxidant enzyme belonging to the peroxiredoxin family of proteins. Prdx1 catalyzes the reduction of H2O2 and alkyl hydroperoxide and plays an important role in different biological processes. Prdx1 also participates in various age-related diseases and cancers. In this study, we investigated the role of Prdx1 in pronephros development during embryogenesis. Prdx1 knockdown markedly inhibited proximal tubule formation in the pronephros and significantly increased the cellular levels of reactive oxygen species (ROS), which impaired primary cilia formation. Additionally, treatment with ROS (H2O2) severely disrupted proximal tubule formation, whereas Prdx1 overexpression reversed the ROS-mediated inhibition in proximal tubule formation. Epistatic analysis revealed that Prdx1 has a crucial role in retinoic acid and Wnt signaling pathways during pronephrogenesis. In conclusion, Prdx1 facilitates proximal tubule formation during pronephrogenesis by regulating ROS levels.

UR - https://www.scopus.com/pages/publications/85051847699

U2 - 10.1038/s41598-017-09262-6

DO - 10.1038/s41598-017-09262-6

M3 - Article

C2 - 28827763

AN - SCOPUS:85051847699

SN - 2045-2322

VL - 7

JO - Scientific Reports

JF - Scientific Reports

IS - 1

M1 - 8874

ER -

Peroxiredoxin1, a novel regulator of pronephros development, influences retinoic acid and Wnt signaling by controlling ROS levels

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