PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Chan Ho Jang; In Ae Lee; Young Ran Ha; Jinkyu Lim; Mi Kyung Sung; Sung Joon Lee; Jong Sang Kim

doi:10.1271/bbb.80079

PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Chan Ho Jang, In Ae Lee, Young Ran Ha, Jinkyu Lim, Mi Kyung Sung, Sung Joon Lee, Jong Sang Kim

School of Food Science & Biotechnology

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

Original language	English
Pages (from-to)	1799-1808
Number of pages	10
Journal	Bioscience, Biotechnology and Biochemistry
Volume	72
Issue number	7
DOIs	https://doi.org/10.1271/bbb.80079
State	Published - 2008

Keywords

Antioxidant
Biomarker
Oxidative stress
Phosphoglycerate kinase 1 (PGK1)

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10.1271/bbb.80079

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title = "PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells",

abstract = "Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.",

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T1 - PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

AU - Jang, Chan Ho

AU - Lee, In Ae

AU - Ha, Young Ran

AU - Lim, Jinkyu

AU - Sung, Mi Kyung

AU - Lee, Sung Joon

AU - Kim, Jong Sang

PY - 2008

Y1 - 2008

N2 - Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

AB - Few protein biomarkers for oxidative stress have been reported. In this study, we attempted to identify the proteins selectively overexpressed in human colon tumor cells by treating with hydrogen peroxide as oxidative stress. A proteomic analysis followed by western blotting showed that phosphoglycerate kinase 1 (PGK1) was induced by hydrogen peroxide in a dose-dependent manner, while its expression was suppressed by a co-treatment with delphinidin, a known antioxidant. Furthermore, several antioxidants, including α-tocopherol, butylated hydroxytoluene (BHT), and Trolox, also inhibited the PGK1 induction caused by hydrogen peroxide. The data suggest that PGK1 might be a potential protein biomarker of intracellular oxidative status.

KW - Antioxidant

KW - Biomarker

KW - Oxidative stress

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SN - 0916-8451

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JO - Bioscience, Biotechnology and Biochemistry

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PGK1 induction by a hydrogen peroxide treatment is suppressed by antioxidants in human colon carcinoma cells

Abstract

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