Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens

Sung Hun Kim, Shukho Kim, Sung Guen Chun, Mi Sun Park, Jeong Hyun Park, Bok Kwon Lee

Research output: Contribution to journalArticlepeer-review

14 Scopus citations

Abstract

We analyzed 66 Salmonella Enteritidis isolates in 2002. Thirty isolates were obtained from human patients with diarrhea, and 36 were obtained from chickens. A total of ten phage types (PT) were identified in the human and chicken isolates. PT1 and PT21 were the predominant PTs in both the human (20% and 13%) and chicken (17% and 47%) isolates. Twelve pulsotypes were generated by PFGE and divided into two major groups. Most of the PFGE types were categorized into cluster group 1. Eighteen chicken isolates in cluster group 1 showed high-level genetic association (>95%) with 22 other human isolates. Additionally, six chicken isolates from cluster group 2 showed fairly high-level genetic association (>95%) with the other seven human isolates. The highest levels of genetic association in humans and chickens were seen with A5-PT21 (11 isolates), A2-PT1 (7 isolates), and B1-PT4 (6 isolates). The Pulsed-Field Gel Electrophoresis (PFGE) and phage typing provided conclusive evidence that human Salmonella infections are attributable to the consumption of contaminated chicken.

Original languageEnglish
Pages (from-to)209-213
Number of pages5
JournalJournal of Microbiology
Volume46
Issue number2
DOIs
StatePublished - Apr 2008

Keywords

  • Phage typing
  • Pulsed-field gel electrophoresis
  • Resistance
  • Salmonella Enteritidis

Fingerprint

Dive into the research topics of 'Phage types and pulsed-field gel electrophoresis patterns of Salmonella enterica serovar Enteritidis isolated from humans and chickens'. Together they form a unique fingerprint.

Cite this