TY - JOUR
T1 - Phenotypic characteristics and karyotype analysis of Hibiscus sabdariffa var. Sabdariffa by fluorescence in situ hybridization (FISH)
AU - Mohammad, Deepo Deen
AU - Mazharul, Islam M.D.
AU - Ann, Tapce Cherry
AU - Kim, Hong Yul
AU - Lim, Ki Byung
N1 - Publisher Copyright:
© 2020 Korean Society for Horticultural Science.
PY - 2020
Y1 - 2020
N2 - The aim of this research was to ascertain the chromosome number, karyomorphology, distribution of 5S and 18S rDNA loci, individual chromosome length, total chromosome length, DNA content, genome sizes, and phenotypic characteristics of H. sabdariffa var. sabdariffa. The chromosome number and detailed karyotype were analyzed by fluorescence in situ hybridization (FISH) using 5S and 18S rDNA probes. H. sabdariffa var. sabdariffa was tetraploid, and the chromosome number of somatic cells was 72 (2n = 4x = 72). According to FISH analysis, four 5S rDNA loci and ten 18S rDNA loci were detected. The lengths of the somatic chromosomes ranged from 3.53 to 6.88 μm, with a total chromosome length of 97.95 μm, where the total length of short arms and long arms were 37.16 and 60.79 μm respectively. The somatic cells of this species had twelve pairs of metacentric (m) and six pairs of submetacentric (sm) chromosomes. The karyotype formula was K = (2n) = 4x = 72 = 48m + 24sm. In this experiment, DNA content and genome sizes of H. sabdariffa var. sabdariffa were estimated by flow cytometry. 2C-DNA content and 2C genome size were 4.29 pg and 3338.8 Mbp, respectively. The leaf shape, leaf apex, and leaf margin were ovate, acute, and palmatipartite, respectively. The flower colour was purple and single in type. In addition, the diameter of the flower was 7.2 cm and the length of the flower eye was 1.87 cm. These results will contribute to further breeding and cytogenetic studies of H. sabdariffa var. sabdariffa.
AB - The aim of this research was to ascertain the chromosome number, karyomorphology, distribution of 5S and 18S rDNA loci, individual chromosome length, total chromosome length, DNA content, genome sizes, and phenotypic characteristics of H. sabdariffa var. sabdariffa. The chromosome number and detailed karyotype were analyzed by fluorescence in situ hybridization (FISH) using 5S and 18S rDNA probes. H. sabdariffa var. sabdariffa was tetraploid, and the chromosome number of somatic cells was 72 (2n = 4x = 72). According to FISH analysis, four 5S rDNA loci and ten 18S rDNA loci were detected. The lengths of the somatic chromosomes ranged from 3.53 to 6.88 μm, with a total chromosome length of 97.95 μm, where the total length of short arms and long arms were 37.16 and 60.79 μm respectively. The somatic cells of this species had twelve pairs of metacentric (m) and six pairs of submetacentric (sm) chromosomes. The karyotype formula was K = (2n) = 4x = 72 = 48m + 24sm. In this experiment, DNA content and genome sizes of H. sabdariffa var. sabdariffa were estimated by flow cytometry. 2C-DNA content and 2C genome size were 4.29 pg and 3338.8 Mbp, respectively. The leaf shape, leaf apex, and leaf margin were ovate, acute, and palmatipartite, respectively. The flower colour was purple and single in type. In addition, the diameter of the flower was 7.2 cm and the length of the flower eye was 1.87 cm. These results will contribute to further breeding and cytogenetic studies of H. sabdariffa var. sabdariffa.
KW - 18S rDNA
KW - 5S rDNA
KW - Breeding
KW - Cytogenetics
KW - Loci
UR - http://www.scopus.com/inward/record.url?scp=85092776317&partnerID=8YFLogxK
U2 - 10.7235/HORT.20200063
DO - 10.7235/HORT.20200063
M3 - Article
AN - SCOPUS:85092776317
SN - 1226-8763
VL - 38
SP - 695
EP - 704
JO - Horticultural Science and Technology
JF - Horticultural Science and Technology
IS - 5
ER -