Phragmites australis (Cav.) Trin. ex Steud. Extract Induces Apoptosis-like Programmed Cell Death in Acanthamoeba castellanii Trophozoites

Hương Giang Lê, Ji Su Choi, Buyng Su Hwang, Yong Tae Jeong, Jung Mi Kang, Tuấn Cường Võ, Pyo Yun Cho, Young Kyung Lee, Won Gi Yoo, Yeonchul Hong, Young Taek Oh, Byoung Kuk Na

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5 Scopus citations

Abstract

Acanthamoeba keratitis (AK) is an infectious ocular disease which is difficult to diagnose correctly and cure. Development of an effective and safe therapeutic drug for AK is needed. Our preliminary screening of more than 200 extracts from wild plants collected in Korea suggested the potential amoebicidal activity of Phragmites australis (Cav.) Trin. ex Steud. extract (PAE) against Acanthamoeba species. Here, we aimed to analyze the amoebicidal activity of PAE on Acanthamoeba and its underlying amoebicidal mechanism. PAE induced amoebicidal activity against both A. castellanii and A. polyphaga trophozoites, while it showed low cytotoxicity in human corneal epithelial cells (HCE-2) and human retinal pigment epithelial cells (ARPE-19). Transmission electron microscopy analysis showed subcellular morphological changes, such as increased granules, abnormal mitochondria, and atypical cyst wall formation, in the PAE-treated A. castellanii. Fluorometric apoptosis assay and TUNEL assay revealed apoptosis-like programmed cell death (PCD) in the PAE-treated A. castellanii. The PAE treatment increased reactive oxygen species production and reduced mitochondrial membrane potential in the amoeba. The enhanced expression of autophagy-associated genes was also detected. These results suggested that PAE exerted a promising amoebicidal effect on A. castellanii trophozoites via the PCD pathway. PAE could be a potential candidate for developing a therapeutic drug for AK.

Original languageEnglish
Article number3459
JournalPlants
Volume11
Issue number24
DOIs
StatePublished - Dec 2022

Keywords

  • Acanthamoeba castellanii
  • amoebicidal activity
  • apoptosis
  • autophagy
  • Phragmites australis (Cav.) Trin. ex Steud. extract
  • programmed cell death

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