Phyllobacterium pellucidum sp. nov., isolated from soil

A bacterial strain, BT25^T, was isolated from soil in Korea. The bacterial cells were Gram-negative and rod-shaped. Phylogenetic analysis using 16S rRNA gene sequences showed that the BT25^T strain was related to the genus Phyllobacterium. BT25^T was 96.6 and 96.5% similar to Phyllobacterium brassicacearum STM 196^T and Phyllobacterium myrsinacearum DSM 5892^T, respectively. The average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) values between BT25^T and the two closest phylogenetic neighbors were calculated to be 78.5 and 77.7, 21.1 and 21.2%, respectively. The major cellular fatty acids were summed feature 8 (C_18:1ω7c/C_18:1ω6c) (29.3%), cyclo-C_19:0ω8c (27.5%), and C_16:0 (16.5%). The BT25^T strain had menaquinone Q-10 as the predominant quinone, as well as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylmonomethylethanolamine, and phosphatidylcholine as the major polar lipids. Based on the phenotypic, phylogenetic, and chemotaxonomic data, the BT25^T strain was classified as a novel Phyllobacterium species. The name Phyllobacterium pellucidum sp. nov. was proposed. The type strain is BT25^T (= KCTC 62765^T = NBRC 114381^T).