TY - JOUR
T1 - Physicochemical characterization and NMR assignments of ginsenosides Rb1, Rb2, Rc, and Rd isolated from Panax ginseng
AU - Cho, Jin Gyeong
AU - Lee, Min Kyung
AU - Lee, Jae Woong
AU - Park, Hee Jung
AU - Lee, Dae Young
AU - Lee, Youn Hyung
AU - Yang, Deok Chun
AU - Baek, Nam In
PY - 2010
Y1 - 2010
N2 - The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides Rb1, Rb2, Rc, and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published1H-and 13C-nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including 1H-1H correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.
AB - The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides Rb1, Rb2, Rc, and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published1H-and 13C-nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including 1H-1H correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.
KW - Diol ginsenoside
KW - Fast atom bombardment/mass spectrometry
KW - High-performance liquid chromatography
KW - Nuclear magnetic resonance
UR - http://www.scopus.com/inward/record.url?scp=79951799760&partnerID=8YFLogxK
U2 - 10.5142/jgr.2010.34.2.113
DO - 10.5142/jgr.2010.34.2.113
M3 - Article
AN - SCOPUS:79951799760
SN - 1226-8453
VL - 34
SP - 113
EP - 121
JO - Journal of Ginseng Research
JF - Journal of Ginseng Research
IS - 2
ER -