Physicochemical characterization and NMR assignments of ginsenosides Rb1, Rb2, Rc, and Rd isolated from Panax ginseng

Jin Gyeong Cho, Min Kyung Lee, Jae Woong Lee, Hee Jung Park, Dae Young Lee, Youn Hyung Lee, Deok Chun Yang, Nam In Baek

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50 Scopus citations

Abstract

The fresh ginseng roots were extracted with aqueous methanol, and the obtained extracts were partitioned using ethyl acetate, n-butanol, and water, successively. The repeated silica gel and octadecyl silica gel column chromatogaraphy for n-butanol fraction afforded four diol ginseng saponins, ginsenosides Rb1, Rb2, Rc, and Rd. The physicochemical, spectroscopic, and chromatographic characteristics of these ginsenosides were measured and compared with those reported in the literature. Some of the peak assignments in previously published1H-and 13C-nuclear magnetic resonance (NMR) spectra were inaccurate. This study employed two-dimensional NMR experiments, including 1H-1H correlation spectroscopy, heteronuclear single quantum correlation, and heteronuclear multiple bond connectivity, to determine exact peak assignments.

Original languageEnglish
Pages (from-to)113-121
Number of pages9
JournalJournal of Ginseng Research
Volume34
Issue number2
DOIs
StatePublished - 2010

Keywords

  • Diol ginsenoside
  • Fast atom bombardment/mass spectrometry
  • High-performance liquid chromatography
  • Nuclear magnetic resonance

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