Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells

Md Badrul Alam; Arif Ahmed; Syful Islam; Hee Jeong Choi; Md Abdul Motin; Sunghwan Kim; Sang Han Lee

doi:10.3390/antiox9111099

Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells

Md Badrul Alam, Arif Ahmed, Syful Islam, Hee Jeong Choi, Md Abdul Motin, Sunghwan Kim, Sang Han Lee

Research output: Contribution to journal › Article › peer-review

22 Scopus citations

Abstract

The antioxidant effects of the ethyl acetate fraction of Dillenia indica bark (DIBEt) and the underlying mechanisms were investigated in tert-butyl hydroperoxide (t-BHP)-stimulated oxidative stress in RAW 264.7 cells. Paper spray ionization-mass spectroscopy with positive-ion mode tentatively revealed 27 secondary metabolites in D. indica bark extract; predominant among them were alkaloids, phenolic acids, and flavonoids. A new triterpenoid (nutriacholic acid) was confirmed in DIBEt for the first time. DIBEt had strong free radical-scavenging capabilities and was also able to reduce t-BHP-induced cellular reactive oxygen species (ROS) generation in RAW 264.7 cells. DIBEt was found to prevent oxidative stress by boosting the levels of heme oxygenase-1 (HO-1) through the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) via the regulation of extracellular signal-regulated kinase (ERK) phosphorylation in RAW 264.7 cells. These results support the potential of DIBEt for defense against oxidative stress-stimulated diseases.

Original language	English
Article number	1099
Pages (from-to)	1-15
Number of pages	15
Journal	Antioxidants
Volume	9
Issue number	11
DOIs	https://doi.org/10.3390/antiox9111099
State	Published - Nov 2020

Keywords

Antioxidant
Dillenia indica
Heme oxygenase 1 (HO-1)
Nuclear factor erythroid 2-related factor 2 (Nrf2)
RAW 264.7 cells

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10.3390/antiox9111099

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Alam, M. B., Ahmed, A., Islam, S., Choi, H. J., Motin, M. A., Kim, S., & Lee, S. H. (2020). Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells. Antioxidants, 9(11), 1-15. Article 1099. https://doi.org/10.3390/antiox9111099

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title = "Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells",

abstract = "The antioxidant effects of the ethyl acetate fraction of Dillenia indica bark (DIBEt) and the underlying mechanisms were investigated in tert-butyl hydroperoxide (t-BHP)-stimulated oxidative stress in RAW 264.7 cells. Paper spray ionization-mass spectroscopy with positive-ion mode tentatively revealed 27 secondary metabolites in D. indica bark extract; predominant among them were alkaloids, phenolic acids, and flavonoids. A new triterpenoid (nutriacholic acid) was confirmed in DIBEt for the first time. DIBEt had strong free radical-scavenging capabilities and was also able to reduce t-BHP-induced cellular reactive oxygen species (ROS) generation in RAW 264.7 cells. DIBEt was found to prevent oxidative stress by boosting the levels of heme oxygenase-1 (HO-1) through the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) via the regulation of extracellular signal-regulated kinase (ERK) phosphorylation in RAW 264.7 cells. These results support the potential of DIBEt for defense against oxidative stress-stimulated diseases.",

keywords = "Antioxidant, Dillenia indica, Heme oxygenase 1 (HO-1), Nuclear factor erythroid 2-related factor 2 (Nrf2), RAW 264.7 cells",

author = "Alam, {Md Badrul} and Arif Ahmed and Syful Islam and Choi, {Hee Jeong} and Motin, {Md Abdul} and Sunghwan Kim and Lee, {Sang Han}",

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doi = "10.3390/antiox9111099",

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volume = "9",

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Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells. / Alam, Md Badrul; Ahmed, Arif; Islam, Syful et al.
In: Antioxidants, Vol. 9, No. 11, 1099, 11.2020, p. 1-15.

Research output: Contribution to journal › Article › peer-review

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AU - Motin, Md Abdul

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AB - The antioxidant effects of the ethyl acetate fraction of Dillenia indica bark (DIBEt) and the underlying mechanisms were investigated in tert-butyl hydroperoxide (t-BHP)-stimulated oxidative stress in RAW 264.7 cells. Paper spray ionization-mass spectroscopy with positive-ion mode tentatively revealed 27 secondary metabolites in D. indica bark extract; predominant among them were alkaloids, phenolic acids, and flavonoids. A new triterpenoid (nutriacholic acid) was confirmed in DIBEt for the first time. DIBEt had strong free radical-scavenging capabilities and was also able to reduce t-BHP-induced cellular reactive oxygen species (ROS) generation in RAW 264.7 cells. DIBEt was found to prevent oxidative stress by boosting the levels of heme oxygenase-1 (HO-1) through the up-regulation of nuclear factor erythroid 2-related factor 2 (Nrf2) via the regulation of extracellular signal-regulated kinase (ERK) phosphorylation in RAW 264.7 cells. These results support the potential of DIBEt for defense against oxidative stress-stimulated diseases.

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KW - Nuclear factor erythroid 2-related factor 2 (Nrf2)

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Phytochemical characterization of dillenia indica l. Bark by paper spray ionization-mass spectrometry and evaluation of its antioxidant potential against t-bhp-induced oxidative stress in raw 264.7 cells

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