Pistacia chinensis methanolic extract attenuated MAPK and Akt phosphorylations in ADP stimulated rat platelets in vitro

Ji Young Park; Mei Hong; Qi Jia; Young Chul Lee; Taddesse Yayeh; Eujin Hyun; Dong Mi Kwak; Jae Youl Cho; Man Hee Rhee

doi:10.1155/2012/895729

Pistacia chinensis methanolic extract attenuated MAPK and Akt phosphorylations in ADP stimulated rat platelets in vitro

Ji Young Park, Mei Hong, Qi Jia, Young Chul Lee, Taddesse Yayeh, Eujin Hyun, Dong Mi Kwak, Jae Youl Cho, Man Hee Rhee

Department of Veterinary Medicine

Research output: Contribution to journal › Article › peer-review

15 Scopus citations

Abstract

Pistacia chinensis (Chinese pistache) is a widely grown plant in southern China where the galls extract is a common practice in folk medicine. However, extracts from this plant have never been attempted for their cardiovascular protective effects in experimental setting. Here therefore we aimed to investigate the antiplatelet activity of Pistacia chinensis methanolic extract (PCME) in ADP stimulated rat platelets in vitro. PCME (2.5-20μ g/mL) inhibited ADP-induced platelet aggregation. While PCME diminished [Ca ²⁺]i, ATP, and TXA2 release in ADP-activated platelets, it enhanced cAMP production in resting platelets. Likewise, PCME inhibited fibrinogen binding to IIb3 and downregulated JNK, ERK, and Akt phosphorylations. Thus, PCME contains potential antiplatelet compounds that could be deployed for their therapeutic values in cardiovascular pathology.

Original language	English
Article number	895729
Journal	Evidence-based Complementary and Alternative Medicine
Volume	2012
DOIs	https://doi.org/10.1155/2012/895729
State	Published - 2012

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title = "Pistacia chinensis methanolic extract attenuated MAPK and Akt phosphorylations in ADP stimulated rat platelets in vitro",

abstract = "Pistacia chinensis (Chinese pistache) is a widely grown plant in southern China where the galls extract is a common practice in folk medicine. However, extracts from this plant have never been attempted for their cardiovascular protective effects in experimental setting. Here therefore we aimed to investigate the antiplatelet activity of Pistacia chinensis methanolic extract (PCME) in ADP stimulated rat platelets in vitro. PCME (2.5-20μ g/mL) inhibited ADP-induced platelet aggregation. While PCME diminished [Ca 2+]i, ATP, and TXA2 release in ADP-activated platelets, it enhanced cAMP production in resting platelets. Likewise, PCME inhibited fibrinogen binding to IIb3 and downregulated JNK, ERK, and Akt phosphorylations. Thus, PCME contains potential antiplatelet compounds that could be deployed for their therapeutic values in cardiovascular pathology.",

author = "Park, {Ji Young} and Mei Hong and Qi Jia and Lee, {Young Chul} and Taddesse Yayeh and Eujin Hyun and Kwak, {Dong Mi} and Cho, {Jae Youl} and Rhee, {Man Hee}",

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AU - Park, Ji Young

AU - Hong, Mei

AU - Jia, Qi

AU - Lee, Young Chul

AU - Yayeh, Taddesse

AU - Hyun, Eujin

AU - Kwak, Dong Mi

AU - Cho, Jae Youl

AU - Rhee, Man Hee

PY - 2012

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AB - Pistacia chinensis (Chinese pistache) is a widely grown plant in southern China where the galls extract is a common practice in folk medicine. However, extracts from this plant have never been attempted for their cardiovascular protective effects in experimental setting. Here therefore we aimed to investigate the antiplatelet activity of Pistacia chinensis methanolic extract (PCME) in ADP stimulated rat platelets in vitro. PCME (2.5-20μ g/mL) inhibited ADP-induced platelet aggregation. While PCME diminished [Ca 2+]i, ATP, and TXA2 release in ADP-activated platelets, it enhanced cAMP production in resting platelets. Likewise, PCME inhibited fibrinogen binding to IIb3 and downregulated JNK, ERK, and Akt phosphorylations. Thus, PCME contains potential antiplatelet compounds that could be deployed for their therapeutic values in cardiovascular pathology.

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Pistacia chinensis methanolic extract attenuated MAPK and Akt phosphorylations in ADP stimulated rat platelets in vitro

Abstract

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