@inbook{b14ad2f1723d4b91b37f1298afd06e3e,
title = "Plant regeneration methods for rapid generation of a large scale Ds transposant population in rice",
abstract = "To mutagenize rice genomes, a two-element system is utilized. This system comprises an immobile Ac element driven by the CaMV 35S promoter, and a gene trap Ds carrying a partial intron with alternative splice acceptors fused to the GUS coding region. Rapid, large-scale generation of a Ds transposant population was achieved using a plant regeneration procedure involving the tissue culture of seed-derived calli carrying Ac and Ds elements. During tissue cultures, Ds mobility accompanies changes in methylation patterns of a terminal region of Ds, where over 70 % of plants contained independent Ds insertions. In the transposon population, around 12 % of plants expressed GUS at the early seedling stage. A flanking-sequence-tag (FST) database has been established by cloning over 19,968 Ds insertion sites and the Ds map shows relatively uniform distribution across the rice chromosomes.",
keywords = "Ac/Ds transposable elements, Gene trap, Mutagenesis, Plant regeneration, Rice, Tissue culture",
author = "Xuan, {Yuan Hu} and Jin Huang and Gihwan Yi and Park, {Dong Soo} and Park, {Soo Kwon} and Eun, {Moo Young} and Yun, {Doh Won} and Lee, {Gang Seob} and Kim, {Tae Ho} and Han, {Chang Deok}",
year = "2013",
doi = "10.1007/978-1-62703-568-2_7",
language = "English",
isbn = "9781627035675",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "101--116",
booktitle = "Plant Transposable Elements",
}