Plum-Derived Exosome-like Nanovesicles Induce Differentiation of Osteoblasts and Reduction of Osteoclast Activation

Yu Seong Park, Hyun Woo Kim, Jin Hyeon Hwang, Jung Young Eom, Dong Ha Kim, Jinho Park, Hyun Jin Tae, Seunghoon Lee, Jae Gyu Yoo, Jee In Kim, Jae Hwan Lim, In Sook Kwun, Moon Chang Baek, Young Eun Cho, Do Kyun Kim

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

Osteoblasts and osteoclasts play crucial roles in bone formation and bone resorption. We found that plum-derived exosome-like nanovesicles (PENVs) suppressed osteoclast activation and modulated osteoblast differentiation. PENVs increased the proliferation, differentiation, and mineralization of osteoblastic MC3T3-E1 cells and osteoblasts from mouse bone marrow cultures. Notably, PENVs elevated the expression of osteoblastic transcription factors and osteoblast differentiation marker proteins in MC3T3-E1 cells. Higher levels of phosphorylated BMP-2, p38, JNK, and smad1 proteins were detected in PENV-treated MC3T3-E1 cells. Additionally, the number of TRAP-positive cells was significantly decreased in PENV-treated osteoclasts isolated from osteoblasts from mouse bone marrow cultures. Importantly, osteoclastogenesis of marker proteins such as PPAR-gamma, NFATc1, and c-Fos were suppressed by treatment with PENVs (50 μg/mL). Taken together, these results demonstrate that PENVs can be used as therapeutic targets for treating bone-related diseases by improving osteoblast differentiation and inhibiting osteoclast activation for the first time.

Original languageEnglish
Article number2107
JournalNutrients
Volume15
Issue number9
DOIs
StatePublished - May 2023

Keywords

  • BMP-2 signaling
  • Runx2
  • bone remodeling
  • exosome-like nanovesicles
  • osteoblasts
  • osteoclasts
  • plum-derived exosome-like nanovesicles (PENVs)

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