TY - JOUR
T1 - Polyphosphoinositides are derived from ether-linked inositol glycerophospholipids in rat brain
AU - Shin, Sun H.
AU - Kim, Jong S.
AU - Kim, Hak R.
AU - Lim, Jin K.
AU - Choi, Byung K.
AU - Yeo, Young K.
PY - 2005
Y1 - 2005
N2 - Membrane inositol glycerophospholipid (IGP) is metabolized to phosphatidylinositol-4-phosphate (PIP), phosphatidylinositol-4, 5-bisphosphate (PIP2), and inositol triphosphate (IP3) in signaling transduction. This study was carried out to determine the subclasses of IGP involved in signaling pathway. The acyl chain moieties of the phospholipids are easily modulated by dietary fatty acids. We analyzed acyl chain composition of IGP 3-subclasses, PIP and PIP2 from rat brain after feeding sunflower seed oil enriched with linoleic acid or fish oil high in eicosapentaenoic acid and docosahexaenoic acid. Long chain polyunsaturated fatty acids (LCPUFA) as eicosapentaenoic acid and docosahexaenoic acid were not incorporated into ether-linked IGP (alkenylacyl-glycerophosphoinositol and alkylacyl- glycerophosphoinositol), PIP and PIP2, while diacyl- glycerophosphoinositol (GPI) contained high LCPUFA. These results suggest that PIP might be phosphorylated from only the ether-linked IGP (alkenylacyl- and alkylacyl species) but not from diacyl subclass for signals to intracellular responses in the plasma membrane of rat brain.
AB - Membrane inositol glycerophospholipid (IGP) is metabolized to phosphatidylinositol-4-phosphate (PIP), phosphatidylinositol-4, 5-bisphosphate (PIP2), and inositol triphosphate (IP3) in signaling transduction. This study was carried out to determine the subclasses of IGP involved in signaling pathway. The acyl chain moieties of the phospholipids are easily modulated by dietary fatty acids. We analyzed acyl chain composition of IGP 3-subclasses, PIP and PIP2 from rat brain after feeding sunflower seed oil enriched with linoleic acid or fish oil high in eicosapentaenoic acid and docosahexaenoic acid. Long chain polyunsaturated fatty acids (LCPUFA) as eicosapentaenoic acid and docosahexaenoic acid were not incorporated into ether-linked IGP (alkenylacyl-glycerophosphoinositol and alkylacyl- glycerophosphoinositol), PIP and PIP2, while diacyl- glycerophosphoinositol (GPI) contained high LCPUFA. These results suggest that PIP might be phosphorylated from only the ether-linked IGP (alkenylacyl- and alkylacyl species) but not from diacyl subclass for signals to intracellular responses in the plasma membrane of rat brain.
KW - Docosahexaenoic acid
KW - Docosapentaenoic acid
KW - Ether-linked inositol glycerophospholipids
KW - Phosphatidylinositol-4,5- bisphosphate
KW - Phosphatidylinositol-4-phosphate
KW - Polyphosphoinositides
UR - http://www.scopus.com/inward/record.url?scp=25444476447&partnerID=8YFLogxK
U2 - 10.5483/bmbrep.2005.38.3.360
DO - 10.5483/bmbrep.2005.38.3.360
M3 - Article
C2 - 15943914
AN - SCOPUS:25444476447
SN - 1225-8687
VL - 38
SP - 360
EP - 365
JO - Journal of Biochemistry and Molecular Biology
JF - Journal of Biochemistry and Molecular Biology
IS - 3
ER -