Portable antigen detector using blue laser diodes and quantum dots

Soon Cheon Karm; Young Gu Ju; Hong Gu Joo

doi:10.1143/JJAP.46.1763

Portable antigen detector using blue laser diodes and quantum dots

Soon Cheon Karm, Young Gu Ju, Hong Gu Joo

Department of Physics Education

Research output: Contribution to journal › Article › peer-review

2 Scopus citations

Abstract

We developed a portable antigen detecting system based on laser illuminated fluorescence microscopy. The introduction of small and reliable semiconductor laser and charge coupled device (CCD) camera enable to make a compact antigen detecting system. The 532 nm green laser used as light sources can excite the 655 and 705 nm quantum dots attached to antibody connecting to target antigen. The light emitted by the excited quantum dots forms an image in CCD camera. The fluorescence image successfully delineates the antigen distribution in the specimen. We demonstrate the same performance using a 405 nm laser diode which can excite wider range of emission wavelength of quantum dots.

Original language	English
Pages (from-to)	1763-1767
Number of pages	5
Journal	Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers
Volume	46
Issue number	4 A
DOIs	https://doi.org/10.1143/JJAP.46.1763
State	Published - 5 Apr 2007

Keywords

Antigen
Confocal microscopy
Laser diode
Quantum dot

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abstract = "We developed a portable antigen detecting system based on laser illuminated fluorescence microscopy. The introduction of small and reliable semiconductor laser and charge coupled device (CCD) camera enable to make a compact antigen detecting system. The 532 nm green laser used as light sources can excite the 655 and 705 nm quantum dots attached to antibody connecting to target antigen. The light emitted by the excited quantum dots forms an image in CCD camera. The fluorescence image successfully delineates the antigen distribution in the specimen. We demonstrate the same performance using a 405 nm laser diode which can excite wider range of emission wavelength of quantum dots.",

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AU - Joo, Hong Gu

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N2 - We developed a portable antigen detecting system based on laser illuminated fluorescence microscopy. The introduction of small and reliable semiconductor laser and charge coupled device (CCD) camera enable to make a compact antigen detecting system. The 532 nm green laser used as light sources can excite the 655 and 705 nm quantum dots attached to antibody connecting to target antigen. The light emitted by the excited quantum dots forms an image in CCD camera. The fluorescence image successfully delineates the antigen distribution in the specimen. We demonstrate the same performance using a 405 nm laser diode which can excite wider range of emission wavelength of quantum dots.

AB - We developed a portable antigen detecting system based on laser illuminated fluorescence microscopy. The introduction of small and reliable semiconductor laser and charge coupled device (CCD) camera enable to make a compact antigen detecting system. The 532 nm green laser used as light sources can excite the 655 and 705 nm quantum dots attached to antibody connecting to target antigen. The light emitted by the excited quantum dots forms an image in CCD camera. The fluorescence image successfully delineates the antigen distribution in the specimen. We demonstrate the same performance using a 405 nm laser diode which can excite wider range of emission wavelength of quantum dots.

KW - Antigen

KW - Confocal microscopy

KW - Laser diode

KW - Quantum dot

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JF - Japanese Journal of Applied Physics, Part 1: Regular Papers and Short Notes and Review Papers

IS - 4 A

ER -

Portable antigen detector using blue laser diodes and quantum dots

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