Abstract
Background: The recombinant vacuolating cytotoxin (rVacA) of Helicobacter pylori that retains native conformational epitopes was evaluated as a vaccine antigen for anti-H. pylori treatment. Methods: s1m1 vacA gene fraction encoding the mature VacA protein was expressed as a soluble protein in E. coli at low temperature. The efficacy of anti-rVacA antibody against VacA or H. pylori was assessed in vitro using AGS cells and in vivo using a murine model. Results: The rabbit antisera against rVacA completely neutralized the vacuolating activity and partially inhibited the cell death induced by VacA in AGS cells. Oral immunization of C57BL/6 mice with rVacA plus CpG-oligodeoxynucleotide (ODN) as an ajuvant stimulated specific anti-VacA antibody and mucosal immune responses which correlated with decreased systemic immune responses and gastric urease activities (p>0.05). Conclusion: The rVacA antigen possessing conformational epitopes may have potential as a vaccine component and may be useful in serological and histopathological analysis.
| Original language | English |
|---|---|
| Pages (from-to) | 2393-2402 |
| Number of pages | 10 |
| Journal | Anticancer Research |
| Volume | 29 |
| Issue number | 6 |
| State | Published - Jun 2009 |
Keywords
- Anti-H. pylori vaccine
- CpG-ODN
- Mucosal immune response
- Neutralizing antibody
- Recombinant VacA
- VacA
