Potential effect of compounds isolated from Coffea arabica against UV-B induced skin damage by protecting fibroblast cells

Yong Hun Cho, Ashutosh Bahuguna, Han Hyuk Kim, Dong in Kim, Hyeon Jeong Kim, Jae Myo Yu, Hyun Gug Jung, Jae Yoon Jang, Jae Hoon Kwak, Geun Hye Park, O. jun Kwon, Young Je Cho, Jeong Yeon An, Cheorun Jo, Sun Chul Kang, Bong Jeun An

Research output: Contribution to journalArticlepeer-review

36 Scopus citations


Ultraviolet (UV) radiation has adverse effects on extracellular matrix (ECM) proteins, leading to formation of wrinkles a hallmark of premature skin aging. The adverse effects of UV radiation are associated with induction of matrix metalloproteinases (MMPs) expression and degradation of collagen and elastin. The present study investigated anti-wrinkle effects of chlorogenic acid (CGA), pyrocatechol (PC) and 3,4,5-tricaffeoyl quinic acid (TCQ), isolated from beans of Coffea arabica, against UV-B stimulated mouse fibroblast cells (CCRF) by measuring expression levels of MMP-1, 3, 9, and type-I procollagen. The three compounds were isolated and purified from coffee grounds using column chromatography and structural examination was evaluated by nuclear magnetic resonance (NMR) analysis. Among the three isolated compounds, CGA effectively suppressed the expression of the MMP-1, 3, and 9 and increased synthesis of type-I procollagen as compared UV-B-stimulated CCRF cells. In addition, CGA dose-dependently inhibited intracellular reactive oxygen species (ROS) production in CCRF cells stimulated by UV radiation. Moreover, CGA displayed a good sun protection factor (SPF) and in vitro DNA damage protection together with inhibition of enzyme xanthine oxidase. The enzyme inhibitory kinetic behavior of CGA was determined by Lineweaver-Burk plot, displayed a mixed type enzyme inhibition with 260.3 ± 4.5 μM, Ki value. The results indicate that CGA has potential to be used as a preventive agent against premature skin aging induced by UV radiation.

Original languageEnglish
Pages (from-to)323-332
Number of pages10
JournalJournal of Photochemistry and Photobiology B: Biology
StatePublished - Sep 2017


  • 3,4,5-Tricaffeoyl quinic
  • Chlorogenic acid
  • MMP-1, 3, 9
  • Pyrocatechol
  • Sun protection factor
  • Type-I procollagen


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