Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus

Ryong Gong; Dae Lee; Jae Lee; Doo Choi; Geum Soog Kim; Sang Lee; Young Seob Lee

doi:10.4103/1995-7645.250339

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus

Ryong Gong, Dae Lee, Jae Lee, Doo Choi, Geum Soog Kim, Sang Lee, Young Seob Lee

School of Life Science and Biotechnology

Rural Development Administration

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.

Original language	English
Pages (from-to)	8-13
Number of pages	6
Journal	Asian Pacific Journal of Tropical Medicine
Volume	12
Issue number	1
DOIs	https://doi.org/10.4103/1995-7645.250339
State	Published - Jan 2019

Keywords

Antimicrobials
Mechanism of action
Polymerase chain reaction
Staphylococcus aureus

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title = "Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus",

abstract = "Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.",

keywords = "Antimicrobials, Mechanism of action, Polymerase chain reaction, Staphylococcus aureus",

author = "Ryong Gong and Dae Lee and Jae Lee and Doo Choi and Kim, {Geum Soog} and Sang Lee and Lee, {Young Seob}",

year = "2019",

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doi = "10.4103/1995-7645.250339",

language = "English",

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T1 - Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus

AU - Gong, Ryong

AU - Lee, Dae

AU - Lee, Jae

AU - Choi, Doo

AU - Kim, Geum Soog

AU - Lee, Sang

AU - Lee, Young Seob

PY - 2019/1

Y1 - 2019/1

N2 - Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.

AB - Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.

KW - Antimicrobials

KW - Mechanism of action

KW - Polymerase chain reaction

KW - Staphylococcus aureus

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JO - Asian Pacific Journal of Tropical Medicine

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ER -

Potentiating activity of rhein in targeting of resistance genes in methicillin-resistant Staphylococcus aureus

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Keywords

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