Abstract
Objective: To investigate the synergistic effect between rhein (RHE) and oxacillin against Staphylococcus aureus (MRSA) at the gene level. Method: A minimum inhibitory concentration and checkerboard dilution test were conducted to evaluate antibacterial activity. Reverse transcriptase polymerase chain reaction was conducted to investigate the gene expressions. Results: RHE exhibited a minimum inhibitory concentration of 62.5-250.0 μg/mL against various MRSA strains and the reference strain, respectively. As revealed by the checkerboard assay, a combination of RHE and oxacillin exhibited synergistic or partially synergistic effects against MRSA strains. RHE decreased the expressions of mecA/blaZ in a dose-dependent manner. RHE also decreased the expressions of the regulator genes mecI/blaI and mecR1/blaR1. Conclusions: We suggest that RHE affects the activity of mecR1/blaR1, which is located in the cell membrane of MRSA and results in the suppression of mecA/mecI/mecR1 and blaZ/blaI/blaR1 gene expressions.
| Original language | English |
|---|---|
| Pages (from-to) | 8-13 |
| Number of pages | 6 |
| Journal | Asian Pacific Journal of Tropical Medicine |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 2019 |
Keywords
- Antimicrobials
- Mechanism of action
- Polymerase chain reaction
- Staphylococcus aureus
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