Presenilin enhancer-2 (PSENEN), a component of the γ-secretase complex, is involved in adipocyte differentiation

S. M. Lee, Y. H. Jeong, H. M. Kim, H. Y. Park, D. Yoon, D. H. Kim, S. Saeki, S. J. Moon, M. J. Kang

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

This study was conducted to identify genes expressed during adipocyte differentiation of bovine intramuscular fibroblast-like cells using differential display reverse-transcriptase polymerase chain reaction. The presenilin enhancer-2 (PSENEN) gene was found to be down-regulated during adipocyte differentiation of bovine intramuscular fibroblast-like cells. The ectopic expression of bovine PSENEN in 3T3-L1 reduced adipogenesis and the inhibition of endogenous PSENEN by siRNA induced adipogenesis on d 4 of adipocyte differentiation of 3T3-L1 cells. Interestingly, the expression of γ-secretase complex gene-related Notch signaling was decreased at d 2 and d 4 during adipocyte differentiation. In addition, expression of the Notch-signaling genes (Notch-1, Hes-1, Pref-1, adipsin) was regulated during adipocyte differentiation by regulation of PSENEN expression. These results suggest that PSENEN plays an important role in adipocyte differentiation and that Notch signaling is involved in adipogenesis. Crown

Original languageEnglish
Pages (from-to)170-180
Number of pages11
JournalDomestic Animal Endocrinology
Volume37
Issue number3
DOIs
StatePublished - Oct 2009

Keywords

  • γ-secretase complex genes
  • Adipocyte differentiation
  • Notch signaling
  • Presenilin enhancer-2

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