TY - JOUR
T1 - Procyanidins from the stem wood of Machilus japonica and their inhibitory effect on LDL oxidation
AU - Park, Hee Jung
AU - Kim, Su Yeon
AU - Song, Na Young
AU - Cho, Jin Gyeong
AU - Kang, Ji Hyun
AU - Jeong, Tae Sook
AU - Lee, Dae Young
AU - Kim, Geum Soog
AU - Kim, Yong Bum
AU - Kang, Hee Cheol
AU - Baek, Nam In
N1 - Publisher Copyright:
© 2013 The Pharmaceutical Society of Korea.
PY - 2014/11
Y1 - 2014/11
N2 - The stem wood of Machilus japonica Siebold & Zucc were extracted with 80 % aqueous MeOH, and the concentrated extract was successively partitioned with ethyl acetate (EtOAc), normal butanol, and water. From the EtOAc fraction, five procyanidins, procyanidin A1 (1), procyanidin A2 (2), procyanidin B7 (3), cinnamtannin B1 (4), and aesculitannin B (5), were isolated. Their chemical structures were identified through spectroscopic data analyses including NMR, MS, and IR. This is the first time any of these compounds have been isolated from this plant. The compounds were evaluated for inhibition activity on LDL oxidation. All of these compounds and the positive control, BHT, showed a very high inhibition effect with IC50 values of 0.94, 2.1, 1.8, 1.1, 1.0, and 1.9 μM, respectively.
AB - The stem wood of Machilus japonica Siebold & Zucc were extracted with 80 % aqueous MeOH, and the concentrated extract was successively partitioned with ethyl acetate (EtOAc), normal butanol, and water. From the EtOAc fraction, five procyanidins, procyanidin A1 (1), procyanidin A2 (2), procyanidin B7 (3), cinnamtannin B1 (4), and aesculitannin B (5), were isolated. Their chemical structures were identified through spectroscopic data analyses including NMR, MS, and IR. This is the first time any of these compounds have been isolated from this plant. The compounds were evaluated for inhibition activity on LDL oxidation. All of these compounds and the positive control, BHT, showed a very high inhibition effect with IC50 values of 0.94, 2.1, 1.8, 1.1, 1.0, and 1.9 μM, respectively.
KW - Low-density lipoprotein
KW - Machilus japonica
KW - Procyanidin
UR - http://www.scopus.com/inward/record.url?scp=84888315407&partnerID=8YFLogxK
U2 - 10.1007/s12272-013-0304-2
DO - 10.1007/s12272-013-0304-2
M3 - Article
C2 - 24297667
AN - SCOPUS:84888315407
SN - 0253-6269
VL - 37
SP - 1403
EP - 1410
JO - Archives of Pharmacal Research
JF - Archives of Pharmacal Research
IS - 11
ER -