Production and characterization of monoclonal antibodies against human ceruloplasmin

Sik Eum Won; Soon Choi Hee; Won Kim Dae; Ho Jang Sang; Hyun Choi Soo; Young Kim So; Jinseu Park; Hoon Kang Jung; Sung Woo Cho; Oh Shin Kwon; Koo Hwang In; Ki Yeon Yoo; Tae Cheon Kang; Ho Won Moo; Young Choi Soo

Production and characterization of monoclonal antibodies against human ceruloplasmin

Sik Eum Won, Soon Choi Hee, Won Kim Dae, Ho Jang Sang, Hyun Choi Soo, Young Kim So, Jinseu Park, Hoon Kang Jung, Sung Woo Cho, Oh Shin Kwon, Koo Hwang In, Ki Yeon Yoo, Tae Cheon Kang, Ho Won Moo, Young Choi Soo

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.

Original language	English
Pages (from-to)	71-76
Number of pages	6
Journal	Journal of Biochemistry and Molecular Biology
Volume	38
Issue number	1
State	Published - 2005

Keywords

Antioxidants
Human ceruloplasmin
Monoclonal antibodies

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This output contributes to the following UN Sustainable Development Goals (SDGs)

Cite this

@article{c3bfed3fea054fc9b0ca24f471c0cca2,

title = "Production and characterization of monoclonal antibodies against human ceruloplasmin",

abstract = "Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.",

keywords = "Antioxidants, Human ceruloplasmin, Monoclonal antibodies",

author = "Won, {Sik Eum} and Hee, {Soon Choi} and Dae, {Won Kim} and Sang, {Ho Jang} and Soo, {Hyun Choi} and So, {Young Kim} and Jinseu Park and Jung, {Hoon Kang} and Cho, {Sung Woo} and Kwon, {Oh Shin} and In, {Koo Hwang} and Yoo, {Ki Yeon} and Kang, {Tae Cheon} and Moo, {Ho Won} and Soo, {Young Choi}",

year = "2005",

language = "English",

volume = "38",

pages = "71--76",

journal = "Journal of Biochemistry and Molecular Biology",

issn = "1225-8687",

publisher = "The Biochemical Society of the Republic of Korea",

number = "1",

}

TY - JOUR

T1 - Production and characterization of monoclonal antibodies against human ceruloplasmin

AU - Won, Sik Eum

AU - Hee, Soon Choi

AU - Dae, Won Kim

AU - Sang, Ho Jang

AU - Soo, Hyun Choi

AU - So, Young Kim

AU - Park, Jinseu

AU - Jung, Hoon Kang

AU - Cho, Sung Woo

AU - Kwon, Oh Shin

AU - In, Koo Hwang

AU - Yoo, Ki Yeon

AU - Kang, Tae Cheon

AU - Moo, Ho Won

AU - Soo, Young Choi

PY - 2005

Y1 - 2005

N2 - Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.

AB - Ceruloplasmin (CP) is the major plasma antioxidant and copper transport protein. Monoclonal antibodies (mAbs) against human CP were produced and characterized. A total of five hybridoma cell lines were established (CP2, CP10, CP20, CP25, CP30). From the epitope mapping analysis, two subgroups of mAbs recognize different peptide fragments were identified. When the purified CP was incubated with the mAbs, the ferroxidase activity of CP was inhibited up to a maximum 57%. Immunoblotting with various tissue homogenates indicated that all the mAbs specifically recognize a single protein band of 130 kDa. They also appear to be extensively cross-reactive among different mammalian including human and avian sources. These results demonstrated that only one type of immunologically similar CP is present in all of the mammalian tissues including human. The CP mAbs could be of great benefit to design the diagnostic kit for CP-related diseases such as Wilson's disease.

KW - Antioxidants

KW - Human ceruloplasmin

KW - Monoclonal antibodies

UR - http://www.scopus.com/inward/record.url?scp=21244463543&partnerID=8YFLogxK

M3 - Article

C2 - 15715949

AN - SCOPUS:21244463543

SN - 1225-8687

VL - 38

SP - 71

EP - 76

JO - Journal of Biochemistry and Molecular Biology

JF - Journal of Biochemistry and Molecular Biology

IS - 1

ER -

Production and characterization of monoclonal antibodies against human ceruloplasmin

Abstract

Keywords

UN SDGs

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